Anti-Haptoglobin antibody (ab52652)
- Product nameAnti-Haptoglobin antibodySee all Haptoglobin primary antibodies ...
- DescriptionMouse polyclonal to Haptoglobin
- Tested applicationsSandwich ELISA, WB more details
- Species reactivityReacts with: Human
Recombinant fusion protein: ICLPSKDYAEVGRVGYVSGWGRNANFKFTDHLKYVMLPVA DQDQCIRHYEGSTVPEKKTPKSPVGVQPILNEHTFCAGMS KYQEDTCYGDAGSAFAVHDL corresponding to amino acids 265-364 of Human Haptoglobin
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 50% Glycerol, Whole serum
- PurityWhole antiserum
- Primary antibody notes This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab52652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||sELISA: Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Haptoglobin (ab85846). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Haptoglobin (ab85846) as Detection.|
|WB||WB: 1/1000. Predicted molecular weight: 45 kDa. This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.|
- FunctionAs a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an Antimicrobial; Antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.
- Tissue specificityExpressed by the liver and secreted in plasma.
- Involvement in diseaseAnhaptoglobinemia (AHP) [MIM:614081]: A condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Congenital haptoglobin deficiency is a risk factor for anaphylactic non-hemolytic transfusion reactions. Note=The disease is caused by mutations affecting the gene represented in this entry.
- Sequence similaritiesBelongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 2 Sushi (CCP/SCR) domains.
- Cellular localizationSecreted.
- Binding peptide antibodyBp antibodyHaptoglobin alpha chain antibody
- Haptoglobin alpha(1S) beta antibodyHaptoglobin alpha(2FS) beta antibodyHaptoglobin beta chain antibodyHaptoglobin, alpha polypeptide antibodyHaptoglobin, beta polypeptide antibodyHP antibodyHp2 alpha antibodyHP2 ALPHA2 antibodyHP2ALPHA2 antibodyHPA1S antibodyHPT antibodyHPT_HUMAN antibodyMGC111141 antibodyZonulin antibody
Anti-Haptoglobin antibody images
All lanes : Anti-Haptoglobin antibody (ab52652) at 1/1000 dilution
Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen
Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (tagged fusion protein)
Lysates/proteins at 20 µg per lane.
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated, at 1/5000 dilution
Predicted band size : 45 kDa