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Overview
Product name
Anti-Haspin antibody
See all Haspin products (3) ...
Description
Rabbit polyclonal to Haspin
Tested applications
Cross reactivity
Predicted to work with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Haspin.
(Peptide available as ab23532.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Phosphorylation
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Western blot - Haspin antibody (ab21686)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21686 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.2 µg/mlDetects a band of approximately 90 kDa (predicted molecular weight: 88 kDa).
Target
Function
Required for normal alignment of chromosomes at metaphase. Phosphorylates histone H3 'Thr-3' during mitosis.
Tissue specificity
Strongly expressed in testis. Also present in thymus and bone marrow and low levels observed in prostate, intestine, lung, spleen and lymph node. Expressed in fetal skin, liver, kidney and small intestine and also in proliferating but not non-proliferating cell lines.
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Haspin subfamily.
Contains 1 protein kinase domain.
Post-translational
modifications
Autophosphorylated on both serine and threonine residues (By similarity). Phosphorylated during mitosis. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ8TF76
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Germ cell-specific gene 2 protein antibody
- GSG2 antibody
- H-Haspin antibody
see all
Database links
- Entrez Gene: 83903 Human
- Omim: 609240 Human
- SwissProt: Q8TF76 Human
- Unigene: 534059 Human
Anti-Haspin antibody images:
Western blot - Haspin antibody (ab21686)
All lanes : Anti-Haspin antibody (ab21686) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2 : HeLa Expressing myc-Haspin (interphase) Whole Cell Lysate - Positive Control at 5 µl
Lane 3 : HeLa Interphase Whole Cell Lysate - Negative Control at 10 µg
Lane 4 : HeLa Expressing myc-Haspin (mitosis) Whole Cell Lysate - Positive Control at 5 µl
Lane 5 : HeLa Mitosis Whole Cell Lysate - Negative Control at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 88 kDa
Observed band size : 90 kDa (why is the actual band size different from the predicted?)
Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 4 minutes
References for Anti-Haspin antibody (ab21686)
This product has been referenced in:
- Varier RAet al. A phospho/methyl switch at histone H3 regulates TFIID association with mitotic chromosomes. EMBO J 29:3967-78 (2010).Read more (PubMed: 20953165) »
- Markaki Yet al. Phosphorylation of histone H3 at Thr3 is part of a combinatorial pattern that marks and configures mitotic chromatin. J Cell Sci 122:2809-19 (2009). WB; Human, Mouse.Read more (PubMed: 19622635) »
See all 3 publications for this product
Publishing research using ab21686? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"