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All lanes : Anti-Hck antibody [3D12E10] (ab63586) at 1/500 dilution
Lane 1 : Immunising protein (recombinant fragment)
Lane 2 : Cos 7 cell lysate transfected with full length Hck-GFP
Predicted band size : 59 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Immunohistochemical analysis of paraffin embedded human colon and pancreas cancer using ab63586 at 1/500 dilution followed by DAB staining.
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