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Western blot - Hck antibody [3D12E10] (ab63586)
![Western blot - Hck antibody [3D12E10] (ab63586)](/ps/datasheet/images/hck.jpg)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hck antibody [3D12E10] (ab63586)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hck antibody [3D12E10] (ab63586)](/ps/datasheet/images/hck2.jpg)
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Product Name
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Hck antibody [3D12E10]
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See all Hck antibodies (6)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [3D12E10] to Hck
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Immunogen
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Ni-NTA purified recombinant Hck-His fusion protein
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
IHC-P: 1/500 - 1/2000.
WB: 1/500 - 1/2000. Detects a band of approximately 95 kDa (predicted molecular weight: 59 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Colon cancer tissue
Pancreatic cancer tissue
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Cellular localization
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Isoform p60-HCK and isoform p59-HCK are associated with membranes. Isoform p60-HCK is also cytoplasmic.
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Research areas
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Immunology >> Immune System Diseases >> Antiviral Signaling
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
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Relevance
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HCK is a tyrosine kinase that is predominantly expressed in hemopoietic cell types. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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3D12E10
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Isotype
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IgG1
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Purity
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Ascites
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Storage buffer
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Preservative: None
Constituents: Ascites
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Form
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Liquid
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Concentration
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Concentration not determined - why is this?
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Hck antibody [3D12E10] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Hck antibody [3D12E10] images, references, products related to ab63586 and other tools.
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Hck antibody [3D12E10] images:
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 Western blot - Hck antibody [3D12E10] (ab63586)
All lanes : Hck antibody [3D12E10] (ab63586) at 1/500 dilution
Lane 1 : Immunising protein (recombinant fragment)
Lane 2 : Cos 7 cell lysate transfected with full length Hck-GFP
Predicted band size : 59 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hck antibody [3D12E10] (ab63586)
Immunohistochemical analysis of paraffin embedded human colon and pancreas cancer using ab63586 at 1/500 dilution followed by DAB staining.
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Search PubMed (MEDLINE) for references to Hck
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Hck antibody [3D12E10] - more information
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Customer reviews (feedback) regarding Hck antibody [3D12E10] |
Customer FAQs regarding Hck antibody [3D12E10] |
Protocols for Hck antibody [3D12E10] |
Price and availability of products related to Hck antibody [3D12E10] |
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Hck antibody [3D12E10] - related products:
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Hck antibody [3D12E10] - other tools:
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Contact Abcam with a Technical Enquiry about Hck antibody [3D12E10] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Western blot - Hck antibody [3D12E10] (ab63586)](/index.html?datasheet=63586#hck.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hck antibody [3D12E10] (ab63586)](/index.html?datasheet=63586#hck2.jpg)
