![Western blot - Hck antibody [3D12E10] (ab63586) image](#hck.jpg)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hck antibody [3D12E10] (ab63586) image](#hck2.jpg)
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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Overview
Product name
Anti-Hck antibody [3D12E10]
See all Hck products (6) ...
Description
Mouse monoclonal [3D12E10] to Hck
Tested applications
WB, IHC-P, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Ni-NTA purified recombinant Hck-His fusion protein
Positive control
Colon cancer tissue Pancreatic cancer tissue
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
3D12E10
Isotype
IgG1
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/2000.Detects a band of approximately 95 kDa (predicted molecular weight: 59 kDa).
IHC-P: 1/500 - 1/2000.
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
Tissue specificity
Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Domain
The SH3 domain mediates binding to HIV-1 Nef.
Cellular localization
Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
Target information above from: UniProt accessionP08631
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Bmk antibody
- Hck 1 antibody
- Hck antibody
see all
Database links
- Entrez Gene: 3055 Human
- Omim: 142370 Human
- SwissProt: P08631 Human
- Unigene: 655210 Human
Anti-Hck antibody [3D12E10] images:
Western blot - Hck antibody [3D12E10] (ab63586)
![Western blot - Hck antibody [3D12E10] (ab63586)](/ps/datasheet/Images/63/ab63586/hck.jpg)
All lanes : Anti-Hck antibody [3D12E10] (ab63586) at 1/500 dilution
Lane 1 : Immunising protein (recombinant fragment)
Lane 2 : Cos 7 cell lysate transfected with full length Hck-GFP
Predicted band size : 59 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hck antibody [3D12E10] (ab63586)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hck antibody [3D12E10] (ab63586)](/ps/datasheet/Images/63/ab63586/hck2.jpg)
Immunohistochemical analysis of paraffin embedded human colon and pancreas cancer using ab63586 at 1/500 dilution followed by DAB staining.
References for Anti-Hck antibody [3D12E10] (ab63586)
ab63586 has not yet been referenced specifically in any publications.
Publishing research using ab63586? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"