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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Overview
Product name
Anti-Hck antibody
See all Hck products (6) ...
Description
Rabbit polyclonal to Hck
Specificity
Recognizes human Hck at 59kD. Weakly recognizes a band at 85kD.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Chicken, Chimpanzee
Immunogen
Synthetic peptide: FLQVGGNTFSKTETS, corresponding to amino acids 29-43 of Human Hck
FLQVGGNTFS KTETS
Positive control
WB: RIPA lysates from HUT-78 cells IP: RIPA lysates from THP-1 monocytic cells
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.01M Tris glycine. pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32860 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.5 - 2 µg/ml.Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
IP: Use at an assay dependent dilution. (Reported to Immunoprecipitate Hck from 1-2 x 10 7 THP-1 monocytic cells lysed in RIPA.)
Target
Function
May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
Tissue specificity
Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Domain
The SH3 domain mediates binding to HIV-1 Nef.
Cellular localization
Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
Target information above from: UniProt accessionP08631
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Bmk antibody
- Bmk antibody
- Hck 1 antibody
see all
Database links
- Entrez Gene: 3055 Human
- Entrez Gene: 15162 Mouse
- Entrez Gene: 25734 Rat
- Omim: 142370 Human
- SwissProt: P08631 Human
- SwissProt: P08103 Mouse
- SwissProt: P50545 Rat
- Unigene: 655210 Human
see all
References for Anti-Hck antibody (ab32860)
ab32860 has not yet been referenced specifically in any publications.
Publishing research using ab32860? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"