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Immunocytochemistry/ Immunofluorescence - Hck (phospho S862) antibody (ab61055)
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Product Name
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Hck (phospho S862) antibody
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See all Hck antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Hck (phospho S862)
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Immunogen
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Synthetic phosphopeptide derived from human Hck around the phosphorylation site of tyrosine 410 (N-E-YP-T-A)
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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Detects target only when phosphorylated at tyrosine 410.
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Tested applications
(see key)
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ELISA, ICC/IF
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/20000.
ICC/IF: 1/500 - 1/1000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cells
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Cellular localization
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Isoform p60-HCK and isoform p59-HCK are associated with membranes. Isoform p60-HCK is also cytoplasmic.
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Research areas
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Immunology >> Immune System Diseases >> Antiviral Signaling
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
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Relevance
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HCK is a tyrosine kinase that is predominantly expressed in hemopoietic cell types. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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The antibody against non-phosphopeptide was removed by chromatography
using non-phosphopeptide corresponding to the phosphorylation site.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Hck (phospho S862) antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Hck (phospho S862) antibody images, references, products related to ab61055 and other tools.
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Hck (phospho S862) antibody images:
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 Immunocytochemistry/ Immunofluorescence - Hck (phospho S862) antibody (ab61055)
1/500-1/1000 ab61055 staining phosphorilated Hck on HeLa cells in the absence (left) or presence (right) of the immunizing phosphopeptide.
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Search PubMed (MEDLINE) for references to Hck
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Hck (phospho S862) antibody - more information
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Hck (phospho S862) antibody - related products:
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Hck (phospho S862) antibody - other tools:
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