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Products:Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
Overview
Product name
Anti-Hck (phospho Y410) antibody
See all Hck products (6) ...
Description
Rabbit polyclonal to Hck (phospho Y410)
Specificity
Detects target only when phosphorylated at tyrosine 410.
Tested applications
ICC/IF, ELISAmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic phosphopeptide derived from human Hck around the phosphorylation site of tyrosine 410 (N-E-YP-T-A)
Positive control
HeLa cells
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Clonality
Polyclonal
Isotype
IgG
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling
Signal Transduction >> Protein Phosphorylation >> Tyrosine Kinases >> Src Family
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Immunocytochemistry/ Immunofluorescence - Hck (phospho Y410) antibody (ab61055)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab61055 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/500 - 1/1000.
ELISA
ELISA: 1/20000ELISA: 1/20000
Target
Function
May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils.
Tissue specificity
Expressed predominantly in cells of the myeloid and B-lymphoid lineages.
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
Domain
The SH3 domain mediates binding to HIV-1 Nef.
Cellular localization
Membrane. Cytoplasm. At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated and Membrane. Membrane > caveola. 20% of this isoform is associated with caveolae, this association depends on palmitoylation of cyteine at position 3.
Target information above from: UniProt accessionP08631
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Bmk antibody
- Hck 1 antibody
- Hck antibody
see all
Database links
- Entrez Gene: 3055 Human
- Entrez Gene: 15162 Mouse
- Entrez Gene: 25734 Rat
- Omim: 142370 Human
- SwissProt: P08631 Human
- SwissProt: P08103 Mouse
- SwissProt: P50545 Rat
- Unigene: 655210 Human
see all
Anti-Hck (phospho Y410) antibody images:
Immunocytochemistry/ Immunofluorescence - Hck (phospho Y410) antibody (ab61055)
1/500-1/1000 ab61055 staining phosphorilated Hck on HeLa cells in the absence (left) or presence (right) of the immunizing phosphopeptide.
References for Anti-Hck (phospho Y410) antibody (ab61055)
ab61055 has not yet been referenced specifically in any publications.
Publishing research using ab61055? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"