HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab14655)

Overview

  • Product nameHeLa (Human epithelial carcinoma cell line) Nuclear LysateSee all HeLa lysates ...
  • General notesWe recommend aliquoting the extracts into single-use fractions and then storing them.
  • Tested applicationsWB, EMSAmore details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.90
    Constituents: 0.48% HEPES, 20% Glycerol, 0.01% Magnesium chloride, 0.077% DTT, 0.004% EGTA, 2.05% Sodium chloride, 1% NP-40
    Note: With Protease inhibitors
  • Concentration information loading...
  • Research Areas
  • BackgroundHeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

Applications

Our Abpromise guarantee covers the use of ab14655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
EMSA Use at an assay dependent dilution. PubMed: 12075007

References for HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab14655)

This product has been referenced in:
  • Weitzel LR  et al. Discovery and verification of protein differences between Er positive/Her2/neu negative breast tumor tissue and matched adjacent normal breast tissue. Breast Cancer Res Treat 124:297-305 (2010). WB . Read more (PubMed: 20087651) »

See 1 Publication for this product

Product Wall

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

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Thank you for contacting us.

I would like to thank you for sending me your protocol, this information is a great help.

I did not recieve any images with you email however. Could you please resend those to me?

I hope t...

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Thank you for contacting Abcam.

I have attached the ppt slide with a red arrow next to the bands that I think are the correct bands(they are the same that you have marked).

There are a few protocol changes that I would recommend to ...

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Thank you for your reply.

I am sorry that we were not able to resolve the issues you were having with ab14655 and I have processed your request for a refund. Your credit note number is ******.

Please let me know if there is anything...

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Thank you for your reply.

The product ab29545 is a whole cell lysate not a nuclear lysate. The only nuclear lysate we have in stock is the ab14655that you currently have.

If you still wish for me to send you ab29545, the whole cell ...

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Thank you for that information.

I do not think adding 4ul more will increase your signal and since you can only load a maximum of 22ul/well, I would like to send you ab29545 as a free of charge replacement. This HeLa cell lysate is already in...

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Thank you for your reply.

I appreciate your patience in this matter, but when you say you loaded 2.5mg/ml of total protein, how many microlitres of lysate is that? I ask, as we send 100ug of total protein at at concentration of 2.5mg/ml (ther...

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Thank you for contacting Abcam.

I am sorry about the problems you have been having with ab14655, HeLa cell lysate. I was wondering if you could tell me how much total protein you loaded of the cell lysate. I ask as there does seem to be a sig...

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Thank you for your enquiry. The nuclear extracts were prepared as detailed in: Dignam, J.D., Lebovitz, R.M. and Roeder, R.G. (1983) Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucl. ...

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Thank you for your enquiry. To prepare a nuclear lysate from HeLa cells I would recommend that you use RIPA buffer as follows. RIPA buffer (RadioImmunoPrecipitation Assay) buffer: RIPA buffer contains the ionic detergent sodium deoxycholate as ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"