General notesWe recommend aliquoting the extracts into single use fractions and then
storing them at -80°C.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.50 Constituent: 10% DTT Note: Note: This lysate was normalised with SDS-PAGE sample buffer to current concentration.
Concentration information loading...
PurityImmunogen affinity purified
Lysate notesThis HeLa Nuclear Extract was prepared using a standard nuclear lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a non-reducing lithium dodecyl sulfate sample loading buffer (LDS).
BackgroundHeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17.
HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.
HeLa (Human epithelial carcinoma cell line) Nuclear Lysate images