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HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)

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Overview

  • Product nameHeLa (Human epithelial carcinoma cell line) Nuclear LysateSee all HeLa lysates ...
  • General notesWe recommend aliquoting the extracts into single use fractions and then storing them at -80°C.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.50
    Constituent: 10% DTT
    Note: Note: This lysate was normalised with SDS-PAGE sample buffer to current concentration.
  • Concentration information loading...
  • Lysate notes This HeLa Nuclear Extract was prepared using a standard nuclear lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a non-reducing lithium dodecyl sulfate sample loading buffer (LDS).
  • Research Areas
  • BackgroundHeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

    • HeLa (Human epithelial carcinoma cell line) Nuclear Lysate images

    • SDS-PAGE - HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)
      SDS-PAGE - HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)
      All lanes  stained with Optiblot Blue (ab119211) (20 ml)

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)

      Lysates/proteins at 10 µg per lane.
    • Western blot - HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)
      Western blot - HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)
      All lanes : Anti-HDAC1 antibody (ab19845) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Observed band size : 60 kDa (why is the actual band size different from the predicted?)


      Exposure time : 2 minutes
    • Western blot - HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)
      Western blot - HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)
      All lanes : Anti-TATA binding protein TBP antibody - ChIP Grade (ab63766) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Observed band size : 45 kDa (why is the actual band size different from the predicted?)


      Exposure time : 2 minutes
    • Western blot - HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)
      Western blot - HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)
      All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Observed band size : 42 kDa (why is the actual band size different from the predicted?)


      Exposure time : 2 minutes
    • Western blot - HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)
      Western blot - HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)
      All lanes : Anti-beta Tubulin antibody - Loading Control (ab21057) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)

      Lysates/proteins at 20 µg per lane.

      Secondary
      Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Observed band size : 51 kDa (why is the actual band size different from the predicted?)


      Exposure time : 20 minutes

    References for HeLa (Human epithelial carcinoma cell line) Nuclear Lysate (ab150036)

    ab150036 has not yet been referenced specifically in any publications.

    Publishing research using ab150036 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"