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HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab29545)

Q&A (13)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • Storage bufferPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • Lysate notes Protein is isolated from HeLa cell pellet using a proprietary technique. The pellet was frozen in liquid nitrogen immediately and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • Research Areas
  • BackgroundHeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17. HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.

    • Applications

    Our Abpromise guarantee covers the use of ab29545 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

    References for HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab29545)

    ab29545 has not yet been referenced specifically in any publications.

    Publishing research using ab29545 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit a Question for ab29545

    Displaying 1 - 10 of 13 results for Q&A

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    We are currently using LAT1 ab85226 for Western blotting, but the signal is really low (even when we have increased the amount of Ab). I was thinking of switching to ab134121 instead. Would we still be measuring the same LAT1 protein? We are measuring it ...

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    Thank you for contacting us.

    Yes, both ab85226 and ab134121 detect the same SLC7A5 (LAT1) protein. http://www.uniprot.org/uniprot/Q01650

    We would guarantee ab134121 to work in WB, IP, and IHC-P with human samples. We know that it does...

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    I am more than happy to try a new vial of p53.  Do you also have a positive control for this antibody that I can also use?  Also the LSS positive control would be very helpful because if that band is truly LSS then that is a good antibody and I would like...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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    Dear recipient,
    Does the Dkk1 antibody ab 61275 include the positive control mentioned
    in the data sheet (HeLa cell lysate)? Or should I purchase it
    separately?
    Thank you very much for your reply.
    Best regards,

    Thank you for contacting us.

    I can confirm that the positive control is not included into an order of the DKK antibody ab61275.

    However, we do offer two products of HeLa cell lysate: ab29545 (tested for WB application) and ab150035 that ...

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    We have just received HeLa Whole Cell Lysate (ab29545). According to datasheet it is recommended to store this product at -80°C but unfortunately the product arrived at room temperature . Only after one day ab29545 was placed to -80°C.

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    Thank you for contacting us.

    We ship many products at 4C so it is not uncommon for this product to be arrived at room temperature. The product will be absolutely fine, please use it without any hesitation.

    I hope this information is help...

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    phone call: - parcel has been left at RT

    Merci de nous avoir contactés.

    Comme discuté au téléphone, nous allons vous envoyer un remplacement gratuit du lysat cellulaire HeLa ab29545. Jeme permets de vous transférer le numéro de commande de rem...

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    I have a question related to that previous order.

    The lysates I bought are not ideal for my purpose, the PEG content is too high for LCMS analysis. And I am afraid of how much protein loss I will incur if trying to remove them from the sample. ...

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    Thank you for contacting us.

    We do not sell cells, only cell lysates. I apologize but we will not be able to provide these in a pre-lysate form.

    Please do not hesitate to contact us if you need any more advice or information.
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    Product code: 2954
    Inquiry: Dear All I am looking for a Cell Line . Is Ab29545 is the right products...? Thank you

    Thank you for contacting us.

    We unfortunately do not sell cell lines.

    The product ab29545 is a cell line lysates for use in western blot.

    I hope this information is helpful to you. Please do not hesitate to contact us if yo...

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    WB with mouse small intestine mucosa
    sees bands at 55 and 70 kDa
    Ab from other company also shows bands at 55 and 70, but also 120 kDa
    Ab: 1/200 o/n
    block: 5% milk
    2nd: HRP, works well
    suggested Hela lysate as positive contro...

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    Thank you for contacting us.

    I have arranged for the shipment of the Hela cell lysate ab29545 on order number xxx.
    Please let me know what results you are obtaining and if this helps to interprete your results with the mouse samples.
    ...

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    Either no signal in WB, or seeing band of interest, plus a non-specific band.

    Thank you for calling Abcam.

    I have sent you a vial of ab29545, HeLa cell lysate to use as a positive control.

    Please let me know if there is anything else I can help you with, or if continue to experience any issue with our antibodies...

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    I have used ab29545 for western blotting with a loading control anti tubulin antibody and there was no signal. I used 3 other cell lysates I purchase from Abcam with the same antibody and they all worked as expected.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab29545.

    To check the status of the order please contact our ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"