Helicobacter pylori IgA Human ELISA Kit (ab108762)
Overview
- Product nameHelicobacter pylori IgA Human ELISA Kit
- Detection methodColorimetric
- Tests1 x 96 test
- Sample typeSerum, Plasma
- Assay typeIndirect
- Sensitivity< 5 NTU/ml
- Range5 NTU/ml - 150 NTU/ml
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
ab108762 Helicobacter pylori IgA Human ELISA Kit is intended for the quantitative determination of IgA class antibodies against Helicobacter pylori in human serum or plasma (citrate).
Helicobacter pylori is a spiral Gram-negative bacterium (2-6.5 µm in size, flagellated) which colonizes the human gastric mucosa. The organism is found in the mucus layer and adheres to the surface mucus epithelium of the stomach but generally does not penetrate the gastric mucosa directly. However, there is a secondary inflammatory response in the mucosa leading to chronic active gastritis. Helicobacter pylori is the primary causative agent in most cases of peptic ulcer disease. Infection rate in Europe is about 30%-40%, worldwide about 50%. There is an inverse relationship between the presence of Helicobacter pylori infection and socioeconomic status. In developing countries, people acquire the infection at an early age such that by young adulthood as many as 90% of the population might have Helicobacter pylori gastritis. In developed western countries the prevalence of Helicobacter pylori gastritis is much lower. Under these conditions, the rate of acquisition is much slower (roughly 1% per annum) and the older one is, the more likely one is to be infected with the organism.
- Tested applicationsIndirect ELISA more details
- PlatformMicroplate
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components Identifier 1 x 96 tests Cover foil 1 unit Helicobacter pylori anti-IgA Conjugate Violet, Black cap 1 x 20ml Helicobacter pylori Coated Wells (IgA) 12 strips of 8 wells 1 unit Helicobacter pylori IgA Additional Control Yellow, Orange cap 1 x 2ml Helicobacter pylori IgA Standard A Blue cap 1 x 2ml Helicobacter pylori IgA Standard B Green Cap 1 x 2ml Helicobacter pylori IgA Standard C Yellow cap 1 x 2ml Helicobacter pylori IgA Standard D Red cap 1 x 2ml IgA Sample Diluent Yellow, White cap 1 x 100ml Stop Solution Red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml Washing Solution (20x conc.) White cap 1 x 50ml - Research Areas
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Alternative names
- H. pylori IgA
Applications
Our Abpromise guarantee covers the use of ab108762 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Indirect ELISA | I-ELISA |
Helicobacter pylori IgA Human ELISA Kit images
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Typical Standard Curve
Protocols
References for Helicobacter pylori IgA Human ELISA Kit (ab108762)
ab108762 has not yet been referenced specifically in any publications.
