Anti-Helicobacter pylori IgG Human ELISA Kit (ab108736)
- Product nameAnti-Helicobacter pylori IgG Human ELISA Kit
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Pos.Serum 19 6.1% Pos.Serum 19 7%
- Tests1 x 96 test
- Sample typeSerum, Plasma
- Assay typeIndirect
- Sensitivity3 U/ml
- Range3 U/ml - 150 U/ml
- Assay durationMultiple steps standard assay
- Species reactivityReacts with: Human
- Product overview
Abcam’s anti-Helicobacter pylori IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against Helicobacter pylori in Human serum and plasma.
A 96-well plate has been precoated with Helicobacter pylori antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized Helicobacter pylori-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Helicobacter pylori IgG sample captured in plate.
- Tested applicationsIndirect ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Washing Solution White cap 1 x 50ml Cover foil 1 unit Helicobacter pylori (IgG) Coated Microplate (12 x 8 wells) 12 strips of 8 wells 1 unit Helicobacter pylori anti-IgG HRP conjugate Blue, Black cap 1 x 20ml Helicobacter pylori IgG Standard A – 0 U/mL Blue Cap 1 x 2ml Helicobacter pylori IgG Standard B – 15 U/mL Green Cap 1 x 2ml Helicobacter pylori IgG Standard C – 75 U/mL Yellow cap 1 x 2ml Helicobacter pylori IgG Standard D – 150 U/mL Red cap 1 x 2ml IgG Sample Diluent Yellow, White cap 1 x 100ml Stop Solution Red cap 1 x 15ml Strip holder 1 unit TMB Substrate Solution Yellow cap 1 x 15ml
- Research Areas
- H. pylori IgG
Our Abpromise guarantee covers the use of ab108736 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Anti-Helicobacter pylori IgG Human ELISA Kit (ab108736)
ab108736 has not yet been referenced specifically in any publications.