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Product name
Anti-Heme oxygenase 2 antibody
See all Heme oxygenase 2 products (7) ...
Description
Goat polyclonal to Heme oxygenase 2
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Cow, Cynomolgus Monkey
Immunogen
Synthetic peptide: DQAGSTLARETLED by a Cysteine residue linker, corresponding to internal sequence amino acids 238-251 of Human Heme oxygenase 2 (NP_002125.3)
DQAGSTLARETLED
Positive control
K562 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Redox metabolism >> Oxidative stress
Metabolism >> Pathways and Processes >> Metabolism processes >> Hypoxia
Cancer >> Cancer Metabolism >> Response to hypoxia
Cell Biology >> Other Antibodies >> Oxidative Stress
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Western blot - Heme oxygenase 2 antibody (ab91515)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab91515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
Sequence similarities
Belongs to the heme oxygenase family.
Contains 2 HRM (heme regulatory motif) repeats.
Cellular localization
Microsome. Endoplasmic reticulum.
Target information above from: UniProt accessionP30519
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Heme oxygenase (decycling) 2 antibody
- Heme oxygenase (decyclizing) 2 antibody
- Heme oxygenase 2 antibody
see all
Database links
- Entrez Gene: 510243 Cow
- Entrez Gene: 3163 Human
- Entrez Gene: 15369 Mouse
- Omim: 141251 Human
- SwissProt: Q2PG53 Cynomolgus Monkey
- SwissProt: P30519 Human
- SwissProt: O70252 Mouse
- Unigene: 284279 Human
see all
Anti-Heme oxygenase 2 antibody images:
Western blot - Heme oxygenase 2 antibody (ab91515)
Anti-Heme oxygenase 2 antibody (ab91515) at 0.3 µg/ml (for 1 hour) + K562 cell lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 36 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
References for Anti-Heme oxygenase 2 antibody (ab91515)
ab91515 has not yet been referenced specifically in any publications.
Publishing research using ab91515? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"