Anti-Hemoglobin antibody (HRP) (ab19362)

What is the predicted MW of the protein. What is the detect MW with ab19362? Do you have an image of the blot?

ANSWER:

I can confirm that the most common hemoglobin type is a tetramer (which contains 4 subunit proteins) called hemoglobin A, consisting of two a and two ß subunits non-covalently bound, each made of 141 and 146 amino acid residues, respectively. This is denoted as a2ß2. The subunits are structurally similar and about the same size. Each subunit has a molecular weight of about 16,000 daltons, for a total molecular weight of the tetramer of about 64,000 daltons.

I have clarified this information on our datasheet and thank you for bringing this to my attention.

Please do not hesitate to contact me if I can be of further assistance,

The problem is that i need an antibody that detects rabbit alpha-globin. Do you think, that one of the other three antibodies is able to do this?

ANSWER:

Thank you for getting back to me.

Unfortunately ab19192, ab19191, and ab19190 have only been shown to demonstrate reactivity against human so far. I do not have details of the predicted reactivity of these antibodies as unfortunately I do not have information as to the location of the epitope. I consider ab19362 your best option as this is predicted to react against rabbit and detects the alpha-globin monomer.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

dear Abcam-team,

I would like to ask, if this antibody is able to detect the alpha-globin monomer?

sincerely

ANSWER:

Thank you for your enquiry.

Yes, Hemoglobin antibody (ab19362) will detect the alpha-globin monomer. However, I have been informed by the lab that our sheep polyclonal antibodies ab19192, ab19191, and ab19190 (all derived from the same sera) all detect the alpha-globin monomer better than ab19362. You may wish to check out the datasheets of each of these antibodies; they are all conjugated to either HRP, AP or FITC.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Is ab19363 the non conjugated version of ab19362?

ANSWER:

I would like to confirm that ab19363 is not the conjugated version of ab19362 ,the two antibodies are very different and unfortunately we do not have an non-HRP version of ab19362, my apologies.

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