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Product Name
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Hemoglobin antibody (HRP)
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See all Hemoglobin antibodies (16)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to Hemoglobin (HRP)
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Immunogen
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Human Hemoglobin
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Reacts with
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Hu
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Predicted to react
(species key)
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Chmp, Rb, RMk
(Due to sequence homology)
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Specificity
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The antibody has been tested in ELISA and IEP with a Human Hemaglobin
Calibrator/Standard but has yet to be tested against endogenous protein.
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Tested applications
(see key)
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ELISA, ICC, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000 - 1/100000.
ICC: 1/200 - 1/500.
WB: 1/1000 - 1/10000.
Colorimetric detection: 1/1000 - 1/10000
Chemiluminescent detection: 1/1000 - 1/30000
Predicted molecular weight: 16 kDa (the protein is a tetramer; each subunit has a molecular weight of 16kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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recombinant human hemoglobin
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Cellular localization
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Secreted
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Research areas
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Cardiovascular >> Blood >> Other
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Relevance
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Hemoglobin is involved in oxygen transport from the lung to the various peripheral tissues.The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult Hemoglobin. The normal adult Hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta zero thalassemia. Reduced amounts of detectable beta globin causes beta plus thalassemia.
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Hemoglobin antibody (HRP) (ab19362) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Conjugation notes
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Molar enzyme/antibody protein ratio is 4:1.
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Storage buffer
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Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.1M Sodium chloride, 50mM HEPES. pH 7.1 Material safety datasheet (MSDS) for this product:
Thimerosal MSDS
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Purification notes
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Antibody concentration was determined by extinction coefficient prior to conjugation: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
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Form
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Liquid
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Conjugation
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Horse Radish Peroxidase
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Hemoglobin antibody (HRP) is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to Hemoglobin
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Hemoglobin antibody (HRP) - more information
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Customer reviews (feedback) regarding Hemoglobin antibody (HRP) |
Customer FAQs regarding Hemoglobin antibody (HRP) |
Protocols for Hemoglobin antibody (HRP) |
Price and availability of products related to Hemoglobin antibody (HRP) |
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Hemoglobin antibody (HRP) - related products:
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Hemoglobin antibody (HRP) - other tools:
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Contact Abcam with a Technical Enquiry about Hemoglobin antibody (HRP) |
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