Anti-Hemopexin antibody (ab133415)
Key features and details
- Rabbit polyclonal to Hemopexin
- Suitable for: WB
- Reacts with: Mouse, Rat
- Isotype: IgG
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Overview
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Product name
Anti-Hemopexin antibody
See all Hemopexin primary antibodies -
Description
Rabbit polyclonal to Hemopexin -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat
Predicted to work with: Rabbit, Dog, Human, Pig, Chimpanzee, Macaque monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human Hemopexin aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab151532) -
Positive control
- This antibody gave a positive signal in the following tissue lysates: Mouse Sciatic Nerve; Rat Sciatic Nerve; Mouse Liver; Rat Liver.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 52 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 72 kDa (predicted molecular weight: 52 kDa). |
Target
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Function
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. -
Tissue specificity
Expressed by the liver and secreted in plasma. -
Sequence similarities
Belongs to the hemopexin family.
Contains 5 hemopexin-like domains. -
Post-translational
modificationsN- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3263 Human
- Entrez Gene: 15458 Mouse
- Entrez Gene: 100174097 Orangutan
- Entrez Gene: 396998 Pig
- Entrez Gene: 58917 Rat
- Omim: 142290 Human
- SwissProt: P02790 Human
- SwissProt: Q91X72 Mouse
see all -
Alternative names
- Beta 1B glycoprotein antibody
- Beta-1B-glycoprotein antibody
- FLJ56652 antibody
see all
Images
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All lanes : Anti-Hemopexin antibody (ab133415) at 1 µg/ml
Lane 1 : Mouse Sciatic Nerve Tissue Lysate
Lane 2 : Rat Sciatic Nerve Rat Tissue Lysate
Lane 3 : Liver (Mouse) Tissue Lysate
Lane 4 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 72 kDa why is the actual band size different from the predicted?
Additional bands at: 12 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
Hemopexin has been shown to migrate at ~70 kDa under reducing conditions by Madore et al PMID: 9930744. We welcome customer feedback. Hemopexin contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab133415 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
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Datasheet download
References (4)
ab133415 has been referenced in 4 publications.
- He Y et al. Exploring Key Genes and Mechanisms in Respiratory Syncytial Virus-Infected BALB/c Mice via Multi-Organ Expression Profiles. Front Cell Infect Microbiol 12:858305 (2022). PubMed: 35586251
- Alves de Souza RW et al. Skeletal muscle heme oxygenase-1 activity regulates aerobic capacity. Cell Rep 35:109018 (2021). PubMed: 33882313
- Nath KA et al. Antithrombotic effects of heme-degrading and heme-binding proteins. Am J Physiol Heart Circ Physiol 318:H671-H681 (2020). PubMed: 32004074
- Hu S et al. Deferoxamine therapy reduces brain hemin accumulation after intracerebral hemorrhage in piglets. Exp Neurol 318:244-250 (2019). PubMed: 31078524