Overview
- Product nameAnti-Hemopexin antibody [32]See all Hemopexin primary antibodies ...
- DescriptionMouse monoclonal [32] to Hemopexin
- SpecificityAb27711 recognises Hemopexin.
- Tested applicationsELISA, Sandwich ELISA more details
- Species reactivityReacts with: Human
- Immunogen
Full length native protein: Hemopexin purified from human plasma. Immunogen was adsorbed onto Al(OH)3 prior to injection in mice.
- General notes
The fusion partner for this antibody is SP2mIL6.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 15mM Sodium Azide.
Constituents: 500mM Sodium chloride, 10mM PBS, pH 7.4. -
Concentration information loading... - PurityProtein A purified
- Primary antibody notes The fusion partner for this antibody is SP2mIL6.
- Clonality Monoclonal
- Clone number32
- IsotypeIgG1
- Light chain typekappa
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab27711 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/20000. |
| Sandwich ELISA | sELISA: Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to Hemopexin (ab48135). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Hemopexin (ab48135) as Detection. |
Target
- FunctionBinds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
- Tissue specificityExpressed by the liver and secreted in plasma.
- Sequence similaritiesBelongs to the hemopexin family.
Contains 5 hemopexin-like domains. - Post-translational
modificationsN- and O-glycosylated. O-glycosylated with core 1 or possibly core 8 glycans. - Cellular localizationSecreted.
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Database links
- Entrez Gene: 3263 Human
- Omim: 142290 Human
- SwissProt: P02790 Human
- Unigene: 426485 Human
Target information above from: UniProt accession
P02790
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Beta 1B glycoprotein antibodyBeta-1B-glycoprotein antibodyFLJ56652 antibody
- HEMO antibodyHEMO_HUMAN antibodyHemopexin antibodyHPX antibodyHX antibody
see all
Anti-Hemopexin antibody [32] images
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Standard Curve for Hemopexin (Analyte: Hemopexin protein (ab98988)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [32] to Hemopexin (ab27711) at 5µg/ml and Detector Antibody Rabbit polyclonal to Hemopexin (ab48135) at 0.5µg/ml.
Protocols
References for Anti-Hemopexin antibody [32] (ab27711)
ab27711 has not yet been referenced specifically in any publications.
![Sandwich ELISA - Hemopexin antibody [32] (ab27711) Sandwich ELISA - Hemopexin antibody [32] (ab27711)](http://a.abcam.com/ps/datasheet/images/27/ab27711/Hemopexin-Primary-antibodies-ab27711-1.jpg)