HepG2 (Human hepatocellular liver carcinoma cell line) Cytoplasmic Lysate (ab14659)

Overview

  • Product nameHepG2 (Human hepatocellular liver carcinoma cell line) Cytoplasmic Lysate
    See all HepG2 lysates
  • General notesWe recommend aliquoting the extracts into single use fractions and then storing them.
  • Tested applicationsWB, EMSAmore details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.90
    Constituents: 0.48% HEPES, 0.75% Potassium chloride, 0.01% Magnesium chloride, 20% Glycerol, 0.009% PMSF, 0.008% DTT
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Research Areas
  • BackgroundHepatitis B virus (HBV) is the major cause of acute and chronic hepatitis, leading to progressive development of necroinflammatory changes in the liver, which can result in cirrhosis and hepatocellular carcinoma. Although the development of an effective vaccine to prevent HBV infection has shown promising results and should lead to its eventual eradication, antiviral chemotherapy remains the only effective method to prevent the progression of the disease in chronic carriers. Therefore, the development of new antiretroviral agents active against HBV is needed. HepG2 cells have an epithelial morphology and are thought to be a very useful model to study HBV virus replication via transfection. Cells are also used for cancer and apoptosis studies (in particular signalling pathway studies).

Applications

Our Abpromise guarantee covers the use of ab14659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
EMSA
  • Application notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for HepG2 (Human hepatocellular liver carcinoma cell line) Cytoplasmic Lysate (ab14659)

    ab14659 has not yet been referenced specifically in any publications.

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    The exact protocol is proprietary for ab14659, but the cytoplasmic membrane is first lysed in the hypotonic buffer to harverst the nuclei. Then the proteins from the nuclei are extracted with a lysis buffer. The sample...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"