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HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)

Q&A (2)

Overview

  • Product nameHepG2 (Human hepatocellular liver carcinoma cell line) Nuclear LysateSee all HepG2 lysates ...
  • General notesWe recommend aliquoting the extracts into single use fractions and then storing them.
  • Tested applicationsEMSA, WB more details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 1mM Magnesium Chloride, 100mM KCl, 0.5mM PMSF, 0.5mM DTT. pH 7.9
  • Concentration information loading...
  • Research Areas
  • BackgroundHepatitis B virus (HBV) is the major cause of acute and chronic hepatitis, leading to progressive development of necroinflammatory changes in the liver, which can result in cirrhosis and hepatocellular carcinoma. Although the development of an effective vaccine to prevent HBV infection has shown promising results and should lead to its eventual eradication, antiviral chemotherapy remains the only effective method to prevent the progression of the disease in chronic carriers. Therefore, the development of new antiretroviral agents active against HBV is needed. HepG2 cells have an epithelial morphology and are thought to be a very useful model to study HBV virus replication via transfection. Cells are also used for cancer and apoptosis studies (in particular signalling pathway studies).

    • Applications

    Our Abpromise guarantee covers the use of ab14660 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    EMSA EMSA: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent dilution.

    References for HepG2 (Human hepatocellular liver carcinoma cell line) Nuclear Lysate (ab14660)

    ab14660 has not yet been referenced specifically in any publications.

    Publishing research using ab14660 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab14660

    Displaying 1 - 2 of 2 results for Q&A

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    Dear Abcam,
    I ordered your monoclonal Anti Tbx 3 Antibody
    ab89220
    and I wonder if you have positive lysate controls, for Western. In that case could you please provide me the catalogue number of these controls?
    Thank you

    Thank you for your technical support and interest in our products.

    As the datasheet indicates, the recommended positive control for ab89220: Anti-Tbx3 antibody is HepG2 cell lysate for Western blot application.

    I have conducted a searc...

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    Thanks for your letter, however you missunderstand me, most probably it is my mistake. I'd like to know how you, at Abcam, are preparing nuclear extract e.g. to produce HeLa nuclear ectract cat# 14655 or Hep G2 nuclear extract cat# 14660. How do you get...

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    Thank you for your enquiry. The nuclear extracts were prepared as detailed in: Dignam, J.D., Lebovitz, R.M. and Roeder, R.G. (1983) Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Nucl. Aci...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"