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HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)

Q&A (9)

Overview

  • Product nameHepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateSee all HepG2 lysates ...
  • General notesCell line: HepG2 (Human hepatocellular carcinoma) Growth media: DMEM & 10% FBS (Fetal bovine serum)
  • Tested applicationsWB more details

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 6.80
    Constituent: 5% Beta mercaptoethanol
  • Concentration information loading...
  • PurityWhole Cell Lysate
  • Lysate notes HepG2 cell lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl flouride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 µg/ml of aprotinin, 5 µg/ml of leupeptin). Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The cell lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
  • Research Areas
  • BackgroundHepatitis B virus (HBV) is the major cause of acute and chronic hepatitis, leading to progressive development of necroinflammatory changes in the liver, which can result in cirrhosis and hepatocellular carcinoma. Although the development of an effective vaccine to prevent HBV infection has shown promising results and should lead to its eventual eradication, antiviral chemotherapy remains the only effective method to prevent the progression of the disease in chronic carriers. Therefore, the development of new antiretroviral agents active against HBV is needed. HepG2 cells have an epithelial morphology and are thought to be a very useful model to study HBV virus replication via transfection. Cells are also used for cancer and apoptosis studies (in particular signalling pathway studies).

    • Applications

    Our Abpromise guarantee covers the use of ab7900 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent concentration. HepG2 cell lysate is ready to load on SDS-PAGE for Western blotting, 20 µg per lane is recommended for mini gel.

    References for HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)

    ab7900 has not yet been referenced specifically in any publications.

    Publishing research using ab7900 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab7900

    Displaying 1 - 9 of 9 results for Q&A

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    Dear Abcam,
    I ordered your monoclonal Anti Tbx 3 Antibody
    ab89220
    and I wonder if you have positive lysate controls, for Western. In that case could you please provide me the catalogue number of these controls?
    Thank you

    Thank you for your technical support and interest in our products.

    As the datasheet indicates, the recommended positive control for ab89220: Anti-Tbx3 antibody is HepG2 cell lysate for Western blot application.

    I have conducted a searc...

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    Hi, I ordered ab7900 to use as a positive control for the primary antibody that I ordered. I was hoping to use the same cell lysate preparation procedure as described in ab7900's datasheet. I noticed that modified RIPA buffer was used with addition of PMS...

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    Thank you for contacting Abcam.

    I believe we spoke on the telephone earlier regarding this question.

    As I mentioned the modified RIP buffer was used as it was found to give slightly more stable results compared to regular RIPA buffer. ...

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    I am interested in some information on your HepG2 Whole Cell Lysate, code ab7900. I see on the website that it comes as a 100µg sample, but I was unable to find the final concentration of material in the storage buffer. What is the volume of storage...

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    Thank you for your inquiry.

    Catalog number ab7900 is a whole HepG2 cell lysate that is provided in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethan...

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    I have purchased ab76122 and it is not working in my western blotting;

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

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    I am searching for a negative control to use with ab47606.

    Thank you for contacting us.

    As a positive control I would recommend using a HepG2 lysate such as ab7900. For a negative control, this same lysate can be de-phosphorylated using this protocol:

    http://www.abcam.com/index.html?pageconfig...

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    Hi,

    I have some problems using the anti-hGNPTAB (Ab108447) and HepG2 (Ab7900) purchased from Abcam (see attached blot). I aliquot and froze the material upon receipt.

    Thank you for contacting Abcam.

    I am sorry about the issues you have been having with ab108447 in western blot. The antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on human. If we cannot resolve th...

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    I accept to test the ab126208 antibody.

    Thank you for the offer.

    I will look forward to receive the new antibody from you soon.

    Best regards

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this ha...

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    I'm very surprised to read that this antibody was never published as applicable in western blot beacuse I chose and bought it for this application.
    I've sent you again the datasheet that I received with the antibody to show you that WB application is...

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    Thank you for contacting us.

    After further investigation, I found that the WB application was added by error which was removed a week after. The reason for removal was, non availability of tested data to prove the suitability. However this does ...

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    Lot number: GR80688-1
    I bought the anti-GPCR GPR128 antibody (ab121649) on 29th March, 2012 to use it in WB applications. I also bought your HepG2 whole cell lysate (ab7900) as a positive control for the antibody. Unfortunatly I couldn't detect the c...

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    Thank you for your email. I am sorry to hear that you have been experiencing difficulties using this antibody.

    We have not tested this antibody before in western blot so we are not sure at what molecular weight the band will be observed. This an...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"