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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - Heparanase 1 antibody (ab59787)
All lanes : Anti-Heparanase 1 antibody (ab59787) at 1/1000 dilution
Lane 1 :
Lane 2 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size : 62 kDa
Observed band size : 57 kDa (why is the actual band size different from the predicted?)
Western blot - Heparanase 1 antibody (ab59787)
Anti-Heparanase 1 antibody (ab59787) + Human epidermis
Predicted band size : 62 kDa
Observed band size : 8,50 kDa (why is the actual band size different from the predicted?)
This image shows the Heparanase 50kDa subunit and the Heparanase 8kDa subunit.
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