Overview
Product nameHepatitis A virus VP1 protein protein
Protein descriptionRecombinant fragment, corresponding to amino acids 502-605 of Hepatitis A virus VP1 protein, 48 kDa.
Expression hostE. coli
Properties
Purity> 90
% by SDS-PAGE
Purification notesab68635 was purified by a proprietary chromatographic technique.
Biological activityab68635 is immunoreactive with sera from HAV-infected individuals.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 10mM Calmodulin Binding buffer (25 mM Tris (pH 8.0). recipe 150 mM NaCl. 1 mM magnesium acetate. 1 mM imidazole), 50% Glycerol, 0.1% SDS, pH 9.6
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab68635
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| WB |
WB: Use at an assay dependent dilution. |
| ELISA |
ELISA: Use at an assay dependent dilution. |
Protein info
RelevanceHepatitis A virus (HAV) is the sole member of the Hepatovirus genus within the family Picornaviridae. The capsid of HAV encloses a single-stranded RNA genome of about 7.5 kb which is translated into a single polyprotein. The virion proteins VP1 to VP4 and the nonstructural proteins are generated from the polyprotein by a cascade of proteolytic cleavages. Only one protease, viral protease 3C, has been implicated in the nine protein scissions. Processing of the capsid protein precursor region generates a unique intermediate, PX (VP1-2A), which accumulates in infected cells and is assumed to serve as precursor to VP1 found in virions, although the details of this reaction have not been determined.
Capsid proteins VP1, VP2, and VP3 form a closed capsid enclosing the viral positive strand RNA genome. VP1 is a major viral antigen.
Cellular localizationCytoplasmic
References for Hepatitis A virus VP1 protein protein (ab68635)
ab68635
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
