Overview
Product nameHepatitis B Virus Surface Antigen (Ayw) protein
Protein descriptionRecombinant full length Hepatitis B Virus Surface Antigen (Ayw) protein cloned from HBV 320 genome expressed in Saccharomyces cerevisae, 24kDa.
Expression hostSaccharomyces cerevisiae
Properties
Purity> 95
% by SDS-PAGE
Purification notesPurity is greater than 98.0% as determined by SDS-PAGE.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 0.2M Sodium chloride, 0.05M PBS, pH 7.2
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab73741
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
| ELISA |
ELISA: Use at an assay dependent dilution. |
Protein info
RelevanceHepatitis B Virus (HBV) infection induces a disease state characterised by liver damage, inflammation and viral persistence. Infection also increases the risk of hepatocellular carcinoma. HBV belongs to the Hepadnaviridae family of viruses. Its genome consists of partially double stranded circular DNA. The DNA is enclosed in a nucleocapsid, or core antigen (HBcAg), which is surrounded by a spherical envelope (surface antigen or HBsAg). The core antigen shares its sequences with the e antigen (HBeAg) but no cross reactivity between the two proteins has been observed. The HBV genome also encodes a DNA polymerase that also acts as a reverse transcriptase.
Hepatitis B infection is normally diagnosed from serological tests that detect HBsAg but as the disease progresses this antigen may no longer be present in the blood and tests for HBcAg are used. If HBsAg can be detected in the blood for longer than six months, chronic hepatitis B is diagnosed.
The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.
Cellular localizationCytoplasmic
References for Hepatitis B Virus Surface Antigen (Ayw) protein (ab73741)
ab73741
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
