Products:Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Hepatitis B
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Greetings! Can ab8635 reconize both glycosylated and non-glycosylated form of HBV Large (39/42Kd), Middle (33/36Kd), and Small (24/27Kd) surface antigen? Thank you very much in advance! Best regards,
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ANSWER: |
I can confirm that the antibody ab8635 recognizes large and middle but not small forms of HBsAg. Both glycosylated and non glycosylated species are reactive. Please do not hesitate to contact us again if you need further information, |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from HEK cells
Lane 2 : Whole cell lysate prepared from HEK cells transfected with pCI M
Lane 3 : Whole cell lysate prepared from HEK cells transfected with pCI M
Lysates/proteins at 50 µg per lane.
Secondary
Sheep anti-mouse HRP conjugated polyclonal at 1/1000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 33,36,39 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 minutes
Image courtesy of Alina Macovei by Abreview.
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