Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)

Product Name 

Hepatitis B Virus pre S2 Antigen antibody [S26]

Product type 

Primary antibodies

Description 

Mouse monoclonal [S26] to Hepatitis B Virus pre S2 Antigen

Immunogen 

Purified serum HBV surface antigen

Specificity 

This antibody reacts with HBV Large and Middle Surface Antigen
but not small forms (both glycosylated and non glycosylated species).

Epitope 

aa positions 131-139 (adw nomenclature)

Tested applications 
(see key)

ELISA, IHC-FoFr, IHC-Fr, IHC-P, IM, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
For western blotting use at 1:1000
For immunohistochemistry use at 1:100

Cellular localization 

Virion membrane (By similarity).

Research areas 

Microbiology >> Organism >> Virus >> DNA Virus >> double stranded DNA Virus >> Hepatitis B

Relevance 

The human hepatitis B virus (HBV) is a DNA virus that causes chronic infections that frequently lead to the development of cirrhosis and cellular hepatocellular carcinoma.
The HBsAg (surface antigen) gene is one long open reading frame but contains three in frame "start" (ATG) codons that divide the gene into three sections, pre-S1, pre-S2, and S. Because of the multiple start codons, polypeptides of three different sizes called large, middle, and small (pre-S1 + pre-S2 + S, pre-S2 + S, or S) are produced.
The preS2 domain is the minimal functional unit of transcription activators that are encoded by the Hepatitis B virus (HBV) surface (S) gene. It is present in more than one-third of the HBV-integrates in HBV induced hepatocarcinoma (HCC).
Pre-S2 is a diagnostically important surface antigen of human hepatitis B virus. The PreS domain (S1 + S2) of Hepatitis B virus (HBV) surface antigen may be a good candidate for an effective vaccine as it activates both B and T cells besides binding to hepatocytes.

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

S26

Myeloma 

Sp2/0

Isotype 

IgG1

Light chain type 

kappa

Purification notes 

Bioreactor harvest: Antibodies generated as bioreactor harvest produce less background as they are less contaminated with other proteins compared to ascites.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Hepatitis B Virus pre S2 Antigen antibody [S26] (ab8635)

  This product has been used in: (two most recent references)

Villet S et al. In vitro characterization of viral fitness of therapy-resistant hepatitis B variants. Gastroenterology 136:168-176.e2 (2009). PubMed: 18996386

Glebe D et al. Pre-s1 antigen-dependent infection of Tupaia hepatocyte cultures with human hepatitis B virus. J Virol 77:9511-21 (2003). PubMed: 12915565
If you publish research using ab8635 please let us know so that we can cite the reference on this datasheet.

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