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Products:Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> Hepatitis A/C/E/G
Overview
Product name
Anti-Hepatitis C Virus E2 antibody [BDI167]
See all Hepatitis C Virus E2 products (3) ...
Description
Mouse monoclonal [BDI167] to Hepatitis C Virus E2
Tested applications
ELISA, IF, WBmore details
Cross reactivity
Reacts with Hepatitis C Virus. Not yet tested in other species.
Immunogen
Recombinant full length HCV E2 protein, genotype 1b
Epitope
Epitope has not been determined.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 10mM PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
BDI167
Isotype
IgG1
Research areas
Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> Hepatitis A/C/E/G
Applications
Show applications key
Our Abpromise guarantee covers the use of ab20852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20 - 1/200.ELISA: 1/20 - 1/200.
IF
IF: 1/10 - 1/50.IF: 1/10 - 1/50.
WB: 1/20 - 1/200.Predicted molecular weight: 1 kDa.( Dilution optimised using Chromogenic detection.)
Target
Relevance
Hepatitis C E2 is a virus envelope glycoprotein which forms a heterodimer with the E1 protein. E2 inhibits human EIF2AK2/PKR activation, preventing the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.
Cellular localization
Viral envelope protein.
Alternative names
- E2 protein antibody
- Envelope glycoprotein E2 antibody
- gp68 antibody
see all
References for Anti-Hepatitis C Virus E2 antibody [BDI167] (ab20852)
This product has been referenced in:
- Ivanov AVet al. Hepatitis C Virus Proteins Activate NRF2/ARE Pathway by Distinct ROS-Dependent and Independent Mechanisms in HUH7 Cells. PLoS One 6:e24957 (2011). WB.Read more (PubMed: 21931870) »
See 1 publication for this product
Publishing research using ab20852? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"