Anti-Hepatitis C Virus E2 antibody (ab20044)

Hello,I am looking for antibodies (preferentially mouseMAb so I can use same secondary), reacting withvirions of hepatitis A virus (HAV), hepatitis C virus (HCV) and parvovirus B19. I have selected ab64295 forB19 and ab8254 for HAV. Could you, please confirm these would detect the intactvirions of B19 and HAV? Also, I am not sure which of your anti-HCV antibodies to choose for this purpose. Could you, please advice?

ANSWER:

Thank you for contacting us.

Following your enquiry I have checked all our HAV, HCV and Parvovirus B19 antibodies and doublechecked with the originators of these if the antibodies would detect intact virions. Thank you for your patience while waiting for the response.

Concerning Parvovirus B19 antibody ab64295:
Indeed, we can confirm that this antibody does detect the intact virus particles. This monoclonal antibody is specific for an epitope common to VP1 and VP2 structural proteins of human Parvovirus B19. These proteins do not need to be purified or the virus does not need to be disrupted in order for the antibody to recognize the target(s).

Concerning Anti-Hepatitis A Virus antibody ab8254:
The Hepatitis A pHM-175 strain was used as the immunogen. The clone MK-01 recognises hepatitis A virus surface antigen, unfortunately no further details are available if this antibody also detects intact virions. However, I am happy to let you know that we have ab1018 available which detects intact Hepatitis A virus:
http://www.abcam.com/index.html?datasheet=1018
http://www.abcam.com/index.html?datasheet=1018.

Concerning Anti-Hepatitis C Virus antibodies: As the envelope glycoproteins, E1 and E2, are essential components of the HCV virion, I have looked for our antibodies against these targets.
ab54555 and ab54556: Both antibodies can detect intact Hepatitis C virions. This has so far only been tested for the inactivated form, unfortunately not yet for non-inactivated virions.
ab20044, ab20852, ab21306 : The target protein recognized by each antibody (HCV E1 or E2) are identified by testing on Western blot. These antibodies have also been tested by ELISA and IF/IHC, but these test methods do not identify if the epitopes are recognized on intact virions. It is therefore not known if these antibodies will recognize intact HCV virions.
http://www.abcam.com/index.html?datasheet=54555 http://www.abcam.com/index.html?datasheet=54555.
http://www.abcam.com/index.html?datasheet=54556 http://www.abcam.com/index.html?datasheet=54556.
http://www.abcam.com/index.html?datasheet=20044 http://www.abcam.com/index.html?datasheet=20044.
http://www.abcam.com/index.html?datasheet=20852 http://www.abcam.com/index.html?datasheet=20852.
http://www.abcam.com/index.html?datasheet=21306 http://www.abcam.com/index.html?datasheet=21306.

Please be reassured that all our products are covered by our Abpromise: We will happily offer Scientific Support in the event that a product is not functioning in the applications and species cited on the product datasheet (and the problem has been reported within 6 months of purchase). If it appears that the antibody is faulty, a replacement, credit or refund will be offered (www.abcam.com/Abpromise).

All tested applications are specified on our datasheets, which are updated as soon as any new information is brought to our attention. If you decide to go ahead and purchase one of these products, please contact our Scientific Support team prior to the purchase as you might be eligible for our testing discount program:

As we do not currently have images for all these antibodies on our datasheets, I can offer a discount off a future purchase if you buy one or more of the products now, use it a tested application and submit an image of your results to us in the form of an Abreview. The discount would be to the value of 1 free primary antibody each.The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Please let me know if you have any questions about this offer and I would be happy to help you further.

I have a question regarding immunogen for ab20044. As it is stated, the immunogen was 'Recombinant full lenght E2 (genotype 1a) protein'. Could you tell me, whether the immunogen was expressed in bacterial or eukaryotic system? I would like to know, if the antibody is able to recognize glycosylated form of E2 in western blot. Also, I would like to ask, if Donkey polyclonal to Goat IgG - H&L, Cy5, ab6566, is suitable for western blot applications.

ANSWER:

Thank you for your enquiry.

I can confrim that the immunogen for ab20044 Hepatitis C Virus E2 antibody is a recombinant antigen expressed in CHO cells. This is a polyclonal antibody and therefore recognizes multiple epitopes on the E2 protein, but we have not tested this antibody for reactivity with glycosylated E2.

With regards to the secondary antibody, ab6566 is not tested in Western Blot. All tested applications are listed on the datasheets which are updated as soon as we recieve any new information. I can recommend a more suitable secondary antibody to use in western blot with ab20044 Hepatitis C Virus E2 antibody is:

ab6741 Rabbit polyclonal to Goat IgG - H&L (HRP) Tested in WB

I can recommend reviewing the online datasheet for further information which I hope will be helpful to you:

http://www.abcam.com/index.html?datasheet=6741

I hope this information is useful to you. Should you have any further questions, please do not hesitate to contact us.