Hepatitis C Virus E2 antibody (ab20044)

Product Name 

Hepatitis C Virus E2 antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to Hepatitis C Virus E2

Immunogen 

Recombinant full length E2 (genotype 1a) protein.

Species Reactivity 

Reacts with Hepatitis C Virus.
Not yet tested in other species.

Specificity 

E2 envelope protein of Hepatitis C Virus.

Tested applications 
(see key)

Conjugation, ELISA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Conjugation: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 0 kDa.

Dilution optimised using Chromogenic detection.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Viral envelope protein.

Research areas 

Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> Hepatitis A/C/E/G

Relevance 

Hepatitis C E2 is a virus envelope glycoprotein which forms a heterodimer with the E1 protein. E2 inhibits human EIF2AK2/PKR activation, preventing
the establishment of an antiviral state. E2 is a viral ligand for CD209/DC-SIGN and CLEC4M/DC-SIGNR, which are respectively found on dendritic cells (DCs), and on liver sinusoidal endothelial cells and macrophage-like cells of lymph node sinuses. These interactions allow capture of circulating HCV particles by these cells and subsequent transmission to permissive cells. DCs are professional antigen presenting cells, critical for host immunity by inducing specific immune responses against a broad variety of pathogens. They act as sentinels in various tissues where they entrap pathogens and convey them to local lymphoid tissue or lymph node for establishment of immunity. Capture of circulating HCV particles by these SIGN+ cells may facilitate virus infection of proximal hepatocytes and lymphocyte subpopulations and may be essential for the establishment of persistent infection.

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

IgG fraction

Storage buffer 

Preservative: 0.1% Sodium Azide
Constituents: 0.01M PBS, pH 7.2

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

>95% pure. Sodium sulfate precipitation and ion-exchange chromatography.

Form 

Liquid

Concentration 

4.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Hepatitis C Virus E2 antibody (ab20044)

  This product has been used in:

Takayama H et al. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3:e2314 (2008). ELISA; Hepatitis C Virus. PubMed: 18523555

If you publish research using ab20044 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Hepatitis C Virus E2 antibody

Customer FAQs regarding Hepatitis C Virus E2 antibody

Protocols for Hepatitis C Virus E2 antibody

Price and availability of products related to Hepatitis C Virus E2 antibody

Hepatitis C Virus E2 antibody - related products: