You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Hepatitis C Virus Genotype 1 NS4 protein (ab67978)

Submit an Abreview Q&A (4)

Overview

  • Product nameHepatitis C Virus Genotype 1 NS4 protein
  • Protein descriptionRecombinant mosaic fragment containing the HCV NS4 immunodominant regions, amino acids 1691-1710, 1712-1733 & 1921-1940.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesab67978 is purified by a proprietary chromatographic technique.
  • Biological activityThis protein is immunoreactive with sera of HCV-infected individuals.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 8.00
    Constituents: 9% Urea, 0.395% Tris HCl, 0.0292% EDTA, 50% Glycerol
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab67978 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution.
    ELISA ELISA: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        HCVHCV NS4Hepatitis C Virus nonstructural antigen 4
        Non structural protein 4ANon structural protein 4BNS4ANS4Bp27p8
      see all
  • RelevanceNS4 A/B are two of the seven nonstructural (NS) proteins making up the HCV polyprotein. The NS proteins recruit the viral genome into an RNA replication complex, which is associated with rearranged cytoplasmic membranes. NS4A acts as a cofactor with the NS3 serine protease and stabilizes its folding. The NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3. HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is a relatively hydrophobic 27-kDa protein. The 4A protein has a molecular weight of 6 kDa.
  • Cellular localizationEndoplasmic reticulum; multi-pass membrane protein.

    • References for Hepatitis C Virus Genotype 1 NS4 protein (ab67978)

    ab67978 has not yet been referenced specifically in any publications.

    Publishing research using ab67978 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab67978 Submit a Question for ab67978

    Displaying 1 - 4 of 4 results for Abreviews and Q&A

    10
    Votes: Highest First

    What is the pH of the buffer the protein is in?

    The buffer is pH 8.0.

    What is the subtype of this antibody? It says HCV Genotype 1 but there are subtypes....

    Thank you for contacting us. The protein is genotype 1b.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase o...

    Read More

    Was this protein produced from the 1a or 1b genotype?
    Will there be an issue with precipitation if we dialyze this into PBS?

    The protein is from genotype 1b, and this will be stable if you dialyse in PBS at 0.4 mg/ml or less.

    What is the concentration of this protein?

    Thank you very much for your call yesterday and for your patience while I've been in touch with the lab.

    The concentration of the lot you have is 1 mg/mL.

    I apologize for the delay in getting you this information,and if you have any fu...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"