Overview
Product nameHepatitis C Virus Genotype 2b NS3 protein
Protein descriptionRecombinant fragment, corresponding to amino acids 1192-1459 of Hepatitis C Virus Genotype 2b, NS3 protein.
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab67964 was purified by proprietary chromatographic techniques.
Biological activityThis protein is immunoreactive with sera of HCV-infected individuals.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 50% Glycerol, 0.2% Triton-X, 1.5M Urea, 25mM Tris HCl, pH 8.0
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab67964
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| WB |
WB: Use at an assay dependent dilution. Predicted molecular weight: 32 kDa. |
| ELISA |
ELISA: Use at an assay dependent dilution. |
Protein info
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Alternative names
HCV 2b NS3HepacivirinHepatitis C virus NS3
see all
RelevanceHCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication.
The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase.
The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein.
HCV Genotype information is important because of the role it plays in predicting HCV medical treatment response and treatment duration. Sustained cure rates (sustained viral response) of 75% or better occur in people with genotypes HCV 2 and 3 in 24 weeks of treatment. Genotypes 1a and 1b, the most prevalent worldwide, have the poorest rates of response to the present treatment regimen, which is a combination of pegylated alfa interferon 2b with ribavirin.
Cellular localizationEndoplasmic reticulum membrane; Peripheral membrane protein (By similarity)
References for Hepatitis C Virus Genotype 2b NS3 protein (ab67964)
ab67964
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
