Overview
Product nameHepatitis C Virus Genotype 5 NS4 protein
Protein descriptionRecombinant mosiac fragment containing the Hepatitis C Virus Genotype 5 NS4 immunodominant regions, amino acids 1691-1710, 1712-1733 and 1921-1940.
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab67986 was purified by a proprietary chromatographic technique.
Biological activityab67986 is immunoreactive with sera of HCV-infected individuals.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 50% Glycerol, 1.5M Urea, 25mM Tris HCl, 1mM EDTA
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab67986
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| WB |
WB: Use at an assay dependent dilution. |
| ELISA |
ELISA: Use at an assay dependent dilution. |
Protein info
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Alternative names
HCVHCV NS4Hepatitis C Virus nonstructural antigen 4
Non structural protein 4ANon structural protein 4B
see all
RelevanceNS4 A/B are two of the seven nonstructural (NS) proteins making up the HCV polyprotein. The NS proteins recruit the viral genome into an RNA replication complex, which is associated with rearranged cytoplasmic membranes. NS4A acts as a cofactor with the NS3 serine protease and stabilizes its folding. The NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3.
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids.
HCV is responsible for a large proportion of worldwide chronic viral hepatitides. Most of these infections develop into chronic hepatitis, which often progresses to liver cirrhosis and hepatocellular carcinoma. At present, (unlike hepatitis A and B), there is no vaccine to prevent hepatitis C infection.
The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is a relatively hydrophobic 27-kDa protein. The 4A protein has a molecular weight of 6 kDa.
Cellular localizationEndoplasmic reticulum; multi-pass membrane protein.
References for Hepatitis C Virus Genotype 5 NS4 protein (ab67986)
ab67986
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
