Overview
Product nameHepatitis C Virus NS3 protein (Beta-Galactosidase)See all Hepatitis C Virus NS3 proteins and peptides ...
Protein descriptionRecombinant fragment, corresponding to amino acids 1450-1643 of Hepatitis C Virus NS3. The protein is fused with beta-galactosidase (114 kDa) at the N-terminus.
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab67975 was purified by proprietary chromatographic techniques.
Biological activityThis protein is immunoreactive with sera of HCV-infected individuals.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 8M Urea, 20mM Tris HCl, 10mM Beta mercaptoethanol, pH 8.0
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab67975
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| WB |
WB: Use at an assay dependent dilution. Predicted molecular weight: 137 kDa. |
| ELISA |
ELISA: Use at an assay dependent dilution. |
Protein info
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Alternative names
HCV NS3 transactivated proteinHepacivirinHepatitis C Virus NS3
Non structural polyprotein NS3NS 3NS3NS3Pp70Serine protease/NTPase/helicase
see all
RelevanceHCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication.
The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase.
The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
Cellular localizationEndoplasmic reticulum membrane
References for Hepatitis C Virus NS3 protein (Beta-Galactosidase) (ab67975)
ab67975
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
