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Hepatitis C Virus NS3 protein (Biotin) (ab68047)

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Overview

  • Product nameHepatitis C Virus NS3 protein (Biotin)See all Hepatitis C Virus NS3 proteins and peptides ...
  • Protein descriptionRecombinant protein containing Hepatitis C Virus NS3 immunodominant regions, a.a. 1450-1643, 22 kDa. Protein is fused with b-galactosidase (114 kDa) at N-terminus.
  • Expression hostE. coli
  • ConjugationBiotin

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesab68047 was purified by a proprietary chromatographic technique.
  • Biological activityab68047 is immunoreactive with sera of HCV-infected individuals.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 1.5M Urea, 25mM Tris HCl, 1mM EDTA, pH 8.0
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab68047 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution.
    ELISA ELISA: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        HCV NS3 transactivated proteinHepatitis C Virus NS3NS 3
        NS3NS3Pp70Serine protease/NTPase/helicase
      see all
  • RelevanceHCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • Cellular localizationEndoplasmic reticulum membrane

    • References for Hepatitis C Virus NS3 protein (Biotin) (ab68047)

    ab68047 has not yet been referenced specifically in any publications.

    Publishing research using ab68047 ? Please let us know so that we can cite the reference in this datasheet

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    Please, what is the most suitable HCV NS3 recombinant protein to use as positive control in WB applications with ab40464?

    Thank you for contacting us.

    Please accept my apologies for the delay, it was a very comprehensive search.

    Unfortunately, I have to let you know that we do not have a suitable HCV NS3 protein available that can be used as a positive co...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"