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Hepatitis C Virus NS3 + NS4A protein (ab84346)

Submit an Abreview Q&A (8)

Overview

  • Product nameHepatitis C Virus NS3 + NS4A protein
  • Protein descriptionFusion protein combined NS4A and NS3 fragments with its protease activity (Hepatitis C Virus (strain NZL1)) with 6X His tag on the N-terminal side of NS4A. Amino acid sequence 1033-1717(Swiss-Prot Q81258).
  • Expression hostE. coli

    • Properties

  • Purification notesab84346 is purified by an affinity column in combination with FPLC chromatography. The purified protein is greater than 95% homogeneous and contains no detectable DNase and RNase activity.
  • Biological activity1 unit equals 1 nanogram of purified protein.
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 20% Glycerol, 20mM Tris Cl, 100mM Potassium chloride, 1mM DTT, 0.2mM EDTA, pH 8.0
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab84346 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS: Use at an assay dependent dilution. for in vitro assay development and screening of protease inhibitors.

    Protein info

    • Alternative names
        Hepatitis C Virus NS3NS3NS3P
        p70Serine protease/NTPase/helicase
      see all

    References for Hepatitis C Virus NS3 + NS4A protein (ab84346)

    ab84346 has not yet been referenced specifically in any publications.

    Publishing research using ab84346 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab84346 Submit a Question for ab84346

    Displaying 1 - 8 of 8 results for Abreviews and Q&A

    10
    Votes: Highest First

    I have one question want to confirm with you.

    We ordered ab 84346 twice from my lab. The first time we got 100ul, this time 10ug, 0.5ug/ul, 20ul.

    Could you check the record and verify for me? Because I need to know for my experiment.

    Thank you for your inquiry.

    According to our records you ordered ab84346 on order 1130723 in July 2012 and received lot GR20464-3, 10ug at 0.5 mg/ml, so 20 ul total. Then ab84346 was reordered on order 1182382 and you received the same lot GR204...

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    We are interested in your proteins, and we need your advises for choosing one of them. We need full-length NS3 (helicase)from HCV, which have adouble strand DNA unwinding activity for our experiments. Is there a NS3 protein with its unwinding activity?

    Following my message from yesterday, I was informed by our source of the NS3/NS4a protein, ab84346, that helicase activity has not been tested.

    I am sorry we could not be of more help. If you have any questions, please contact us. Good luck in ...

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    We are interested in your proteins, and we need your advises for choosing one of them. We need full-length NS3 (helicase) from HCV, which have a double strand DNA unwindingactivity for our experiments (please refer attached file). Is there a NS3 protein w...

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    Thank you for contacting us.

    The product ab84346 has not been tested for helicase activity but I have asked our source for the protein to confirm. I will forward what I receive from them.

    We do not have a full-length NS3. There is an...

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    I read it. Even this is not exactly what described about ab84346. But if it is really as you said in this email, it should work for me.

    I care about the NS3. It seems NS3 is full length. I want to test helicase. I do not wish NS3 is short ...

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    Thank you for your reply.

    Yes, this protein will have both the Helicase ATP-binding (a.a. 1223 - 1375) and Helicase C-terminal (a.a. 1382 - 1544) domains.

    http://www.uniprot.org/uniprot/Q81258

    I apologize for the confusion, a...

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    Is this the Hepatitis C Virus NS3 protein Recombinant full length Hepatitis C Virus (strain NZL1) NS3 protein, or HCV NS3/4A protease?

    Thank you for your inquiry.

    I heard back from the lab that this product is a fusion protein combined NS4A and NS3 fragments with it protease activity. There is a 6x His tag on the N-terminal side of NS4A.

    http://www.uniprot.org/uniprot...

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    Would like to test this antibody in a helicase activity assay.

    DISCOUNT CODE: ***
    Expiration date: November 19, 2012

    This code will give you 1 free protein before the expiration date. To redeem this offer, please submit an Abreview forab84346 in a helicase functionalstudyand include this code in the &l...

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    Please, what is the most suitable HCV NS3 recombinant protein to use as positive control in WB applications with ab40464?

    Thank you for contacting us.

    Please accept my apologies for the delay, it was a very comprehensive search.

    Unfortunately, I have to let you know that we do not have a suitable HCV NS3 protein available that can be used as a positive co...

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    Dear Abcam,

    I was wondering if your recombinant HCV NS3 protein (ab84346 or ab91395) contains an additional N-terminal methionine or if it is the wild-type sequence starting with an alanine? Since it is recombinant I assume you have added M in ...

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    Thank you for your enquiry and for your patience.

    I can confirm that both these recombinant HCV NS3 proteins (ab84346 and ab91395) contain an additionalN terminal Methionine.

    I hope this will be helpful to you. If you have any furthe...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"