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Hepatitis C Virus NS3 protein (ab91395)

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Overview

  • Product nameHepatitis C Virus NS3 proteinSee all Hepatitis C Virus NS3 proteins and peptides ...
  • Protein descriptionRecombinant fragment (Hepatitis C Virus), subtype 1c (1192 - 1459aa) with a 6x His tag.
  • Protein length268 amino acids
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesab91395 is purified by proprietary chromatographic techniques. Purity: >95% by SDS-PAGE.
  • Biological activityab91395 is immunoreactive with sera of HCV-infected individuals.
  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 0.2% Triton-X-100, 1.5M Urea, 25mM Tris HCl, pH 8
  • Concentration information loading...
  • Sequence notesab91395 contains HCV NS3 immunodominant region (1192-1459aa).
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91395 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    WB
    ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Hepatitis C Virus NS3NS3NS3P
        p70Serine protease/NTPase/helicase
      see all
  • RelevanceHCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • Cellular localizationEndoplasmic reticulum membrane

    • References for Hepatitis C Virus NS3 protein (ab91395)

    ab91395 has not yet been referenced specifically in any publications.

    Publishing research using ab91395 ? Please let us know so that we can cite the reference in this datasheet

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    Dear Abcam,

    I was wondering if your recombinant HCV NS3 protein (ab84346 or ab91395) contains an additional N-terminal methionine or if it is the wild-type sequence starting with an alanine? Since it is recombinant I assume you have added M in ...

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    Thank you for your enquiry and for your patience.

    I can confirm that both these recombinant HCV NS3 proteins (ab84346 and ab91395) contain an additionalN terminal Methionine.

    I hope this will be helpful to you. If you have any furthe...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"