You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Hepatitis C Virus NS4 protein (Tagged) (ab49029)

Submit an Abreview Q&A (3)

Overview

  • Product nameHepatitis C Virus NS4 protein (Tagged)See all Hepatitis C Virus NS4 proteins and peptides ...
  • Protein descriptionRecombinant HCV NS4, A plus B proteins, corresponding to amino acids 1658-1863 of the HCV polyprotein, 19 kDa. Fused to beta-Galactosidase at the N terminus. Fluorescein conjugated.
  • Expression hostE. coli
  • ConjugationFITC

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activityReacts strongly with human HCV positive serum.
  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 8M Urea, 20mM Tris HCl, 10mM Beta mercaptoethanol, pH 8.0
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab49029 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    WB WB: Use at an assay dependent dilution.
    ELISA ELISA: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        HCVHCV NS4Hepatitis C Virus nonstructural antigen 4
        Non structural protein 4ANon structural protein 4BNS4ANS4Bp27p8
      see all
  • RelevanceNS4 A/B are two of the seven nonstructural (NS) proteins making up the HCV polyprotein. The NS proteins recruit the viral genome into an RNA replication complex, which is associated with rearranged cytoplasmic membranes. NS4A acts as a cofactor with the NS3 serine protease and stabilizes its folding. The NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3. HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. HCV is responsible for a large proportion of worldwide chronic viral hepatitides. Most of these infections develop into chronic hepatitis, which often progresses to liver cirrhosis and hepatocellular carcinoma. At present, (unlike hepatitis A and B), there is no vaccine to prevent hepatitis C infection. The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is a relatively hydrophobic 27-kDa protein. The 4A protein has a molecular weight of 6 kDa.
  • Cellular localizationEndoplasmic reticulum; multi-pass membrane protein.

    • References for Hepatitis C Virus NS4 protein (Tagged) (ab49029)

    ab49029 has not yet been referenced specifically in any publications.

    Publishing research using ab49029 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab49029 Submit a Question for ab49029

    Displaying 1 - 3 of 3 results for Abreviews and Q&A

    10
    Votes: Highest First

    protocol to use protein in ELISA?


    The ELISA protocol is below.
    If antigen is diluted at least 1 to 10 the 8M Urea will not be a problem.


    ELISA protocol

    1. Dilute HCV antigen to 0.1 mg/25 ml (4.0 mg/ml) in coating buffer (0.01M Na2CO3; 0.01M Na3EDTA; 0...

    Read More

    protocol to use protein in ELISA?


    The lab let me know that they used a standard ELISA protocol with 50 microgram per well as a starting concentration with serial dilutions. The urea did not interfere.



    Inquiry: 1. Does anibody ab124526 react with antigen ab49029? 2. How many biotin molecules have ab 124526

    Thank you for contacting us. In theory the protein ab49029 should react with antibidy ab124526, since the protein is full length Hepatitis C Virus NS4 protein. However, this has not been tested and there is a possibility the tag could prevent the antibody...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"