Overview
Product nameHepatitis C Virus NS4 proteinSee all Hepatitis C Virus NS4 proteins and peptides ...
Protein descriptionThe E.Coli derived 66kDa recombinant HCV NS4 Mosaic protein is an artificial mosaic polypeptide composite constructed from diagnostically relevant antigenic regions derived from the NS4 region of different HCV genotypes.The protein contains region 5-1-1 and region 59 containing sequence 1789 - 1867 aa and sequence 2322 - 2423 aa of the HCV NS4 genotype lb and 10 other diagnostically relevant antigenic regions derived from the NS4 region of different HCV genotypes.
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab67977 is purified by a proprietary chromatographic technique.
Biological activityThis protein is immunoreactive with sera of HCV-infected individuals.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 50mM Tris, 5mM EDTA, pH 8.0
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab67977
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| WB |
|
| ELISA |
|
Application notesELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Runs as a band at approximately 66 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
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Alternative names
HCVHCV NS4Hepatitis C Virus nonstructural antigen 4
Non structural protein 4ANon structural protein 4BNS4ANS4Bp27p8
see all
RelevanceNS4 A/B are two of the seven nonstructural (NS) proteins making up the HCV polyprotein. The NS proteins recruit the viral genome into an RNA replication complex, which is associated with rearranged cytoplasmic membranes. NS4A acts as a cofactor with the NS3 serine protease and stabilizes its folding. The NS3-NS4A complex is essential for the activation of the latter and allows membrane anchorage of NS3.
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids.
HCV is responsible for a large proportion of worldwide chronic viral hepatitides. Most of these infections develop into chronic hepatitis, which often progresses to liver cirrhosis and hepatocellular carcinoma. At present, (unlike hepatitis A and B), there is no vaccine to prevent hepatitis C infection.
The hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is a relatively hydrophobic 27-kDa protein. The 4A protein has a molecular weight of 6 kDa.
Cellular localizationEndoplasmic reticulum; multi-pass membrane protein.
References for Hepatitis C Virus NS4 protein (ab67977)
ab67977
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
