Overview
Product nameHepatitis C Virus genotype 1a NS5 proteinSee all Hepatitis C Virus genotype 1a NS5 proteins and peptides ...
Protein descriptionRecombinant fragment, corresponding to amino acids 2212-2313 of Hepatitis C Virus, genotype 1a, NS5 protein.
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesThis protein was purified by proprietary chromatographic techniques.
Biological activityThis protein is immunoreactive with sera of HCV-infected individuals.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 50% Glycerol, 0.2% Triton-X, 1.5M Urea, 25mM Tris HCl, pH 8
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab68373
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| WB |
WB: Use at an assay dependent dilution. Predicted molecular weight: 12 kDa. |
| ELISA |
ELISA: Use at an assay dependent dilution. |
Protein info
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Alternative names
HCV 1a NS5Hepatitis C virus NS5ANon-structural protein 5Ap56
RelevanceHepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008).
NS5B (non-structural protein 5B) is an RNA-dependant RNA polymerase responsible for replication of the hepatitis C viral genome, and is currently a principal target for chemotherapeutic inhibition of HCV replication.
Hepatitis C virus (HCV) can cause chronic hepatitis, cirrhosis and hepatocellular carcinoma. At present there is no vaccine effective against HCV.
Cellular localizationEndoplasmic reticulum membrane
References for Hepatitis C Virus genotype 1a NS5 protein (ab68373)
ab68373
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
