Overview
Product nameHepatitis C Virus genotype 2a NS5 proteinSee all Hepatitis C Virus genotype 2a NS5 proteins and peptides ...
Protein descriptionRecombinant fragment, corresponding to amino acids 2212-2313 of Hepatitis C Virus, genotype 2a, NS5 protein, containing the immunodominant regions.
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesThis protein was purified by proprietary chromatographic techniques.
Biological activityThis protein is immunoreactive with sera of HCV-infected individuals.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 50% Glycerol, 0.2% Triton-X, 1.5M Urea, 25mM Tris HCl, pH 8
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab68375
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| WB |
WB: Use at an assay dependent dilution. |
| ELISA |
ELISA: Use at an assay dependent dilution. |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
Protein info
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Alternative names
HCV NS5Hepatitis virus NS5ANon structural protein 5A
see all
RelevanceHCV is classified into the genus Hepacivirus of the family Flaviviridae. Like all the members of the family, HCV is an enveloped, single-stranded, positive-sense RNA virus. Its genome (about 9600 nt) is flanked at both termini by conserved, highly structured non-translated regions (NTRs) and encodes a polyprotein precursor (about 3000 aa), which is proteolytically processed by host and viral proteases to produce the structural (core, E1, E2 and p7) and non-structural (NS2, NS3, NS4A, NS4B, NS5A and NS5B) proteins of the virus. Recently, an additional protein has been identified, whose function remains unknown.
HCV is a major cause of acute hepatitis and chronic liver disease, including cirrhosis and liver cancer. Currently, no vaccine is currently available to prevent hepatitis C, and therapeutic approaches to treatment are still limited.
NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008).
NS5B RNA-dependant RNA polymerase is responsible for replication of the hepatitis C viral genome, and is currently a principal target for chemotherapeutic inhibition of HCV replication.
References for Hepatitis C Virus genotype 2a NS5 protein (ab68375)
ab68375
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
