Overview
Product nameHepatitis C Virus genotype 6a NS5 protein
Protein descriptionRecombinant fragment, corresponding to amino acids 2212-2313 of Hepatitis C Virus NS5 protein, Genotype 6a.
Swiss Prot Ids: Q5I2N3 (isolate 6a33) and O39927 (isolate EUHK2).
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab68377 was purified by a proprietary chromatographic technique.
Biological activityab68377 is immunoreactive with sera of HCV-infected individuals.
FormLiquid
Storage instructionsStore at -20°C.
Storage bufferPreservative: None
Constituents: 50% Glycerol, 0.2% Triton-X 1.5M Urea, 25mM Tris HCl, pH 8
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab68377
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| WB |
WB: Use at an assay dependent dilution. Predicted molecular weight: 12 kDa. |
| ELISA |
ELISA: Use at an assay dependent dilution. |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
Protein info
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Alternative names
HCV NS5Hepatitis C virus non structural protein 5Hepatitis C virus nonstructural protein 5
Hepatitis C Virus NS5NS5Ap56
see all
RelevanceHepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Several different genotypes of HCV with slightly different genomic sequences have since been identified that correlate with differences in response to treatment with interferon alpha.
NS5A is a ~56 kDa pleiotropic protein with key roles in both viral RNA replication and modulation of the physiology of the host cell. It's exact role is not currently known (2008).
NS5B (non-structural protein 5B) is an RNA-dependant RNA polymerase responsible for replication of the hepatitis C viral genome, and is currently a principal target for chemotherapeutic inhibition of HCV replication.
Cellular localizationEndoplasmic reticulum membrane
References for Hepatitis C Virus genotype 6a NS5 protein (ab68377)
ab68377
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
