Overview
Product nameHepatitis C virus Genotype 1b NS3 protein
Protein descriptionRecombinant fragment, corresponding to amino acids 1359-1456 of Hepatitis C virus Genotype 1b NS3; 98 amino acids, MWt 10.4 kDa (UniProt P26662).
Expression hostE. coli
Properties
Purity> 95
% by SDS-PAGE
Purification notesab91470 is purified by proprietary chromatographic techniques. Purity >95% by SDS-PAGE and RP-HPLC
Biological activityImmunoreactive with sera of HCV-infected individuals.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: 50% Glycerol, 1.5M Urea, 25mM Tris HCl, 1mM EDTA, pH 7.5
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab91470
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| WB |
|
| ELISA |
|
Application notesELISA: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
-
Alternative names
Serine protease/NTPase/helicase NS3
RelevanceThe nonstructural protein NS3 of the hepatitis C virus (HCV) is indispensable for virus replication and a multifunctional enzyme that contains three catalytic activities such as serine protease, helicase, and NTPase. The N-terminal domain of the protein contains protease
activity and the C-terminal domain contains nucleotide triphosphatase and RNA helicase activity. It has been shown that NS2/3 cleavage is mediated by NS2-3 protease, whereas NS3 serine protease is responsible for the other four cleavage sites of the nonstructural (NS) region.
Immunoblot analysis on serum samples from 90 patients with chronic hepatitis C virus infection revealed four putative immunogenic regions within the NS3 protein of the virus: E (around amino acids 1250/1251), A (within amino acids 1250-1334), A/B (around amino acids 1323 and 1334), and B/C (around amino acids 1407 and 1412). Among them, region E was most immunodominant, and region A was recognized much less frequently by patients with cirrhosis than by those with chronic hepatitis.
Cellular localizationHost endoplasmic reticulum membrane; Peripheral membrane protein. Note: NS3 is associated to the ER membrane through its binding to NS4A.
References for Hepatitis C virus Genotype 1b NS3 protein (ab91470)
ab91470
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
