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Products:Cardiovascular >> Blood >> Serum Proteins
Overview
Product name
Anti-Hepcidin-25 antibody
See all Hepcidin-25 products (8) ...
Description
Rabbit polyclonal to Hepcidin-25
Tested applications
ICC/IF, WB, IHC-Fr, IHC-Pmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Hepcidin-25.
(Peptide available as ab31875.)
Positive control
HAMP Human Full-length Recombinant Protein (Tagged)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Blood >> Erythrocytes
Signal Transduction >> Metabolism >> Vitamins / Minerals
Cardiovascular >> Blood >> Serum Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab30760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution. (PubMed: 19059216)
WB: Use a concentration of 1 µg/mlDetects a band of approximately 32 kDa (predicted molecular weight: 3 kDa).
IHC-Fr: 1/200
IHC-P: Use a concentration of 1 - 5 µg/ml.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Target
Function
Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages.
Has strong antimicrobial activity against E.coli ML35P N.cinerea and weaker against S.epidermidis, S.aureus and group b streptococcus bacteria. Active against the fungus C.albicans. No activity against P.aeruginosa.
Tissue specificity
Highest expression in liver and to a lesser extent in heart and brain. Low levels in lung, tonsils, salivary gland, trachea, prostate gland, adrenal gland and thyroid gland. Secreted into the urine.
Involvement in disease
Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B) [MIM:613313]; also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy.
Sequence similarities
Belongs to the hepcidin family.
Cellular localization
Secreted.
Target information above from: UniProt accessionP81172
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Form
Hepcidin produces a propeptide of 84 amino acids that undergoes enzymatic cleavage into mature peptides of 20, 22, and 25 amino acids. Active peptides are rich in cysteines that form intramolecular bonds and stabilize the beta-sheet structure
Alternative names
- Hamp antibody
- HEPC antibody
- HEPC_HUMAN antibody
see all
Database links
- Entrez Gene: 57817 Human
- Entrez Gene: 84604 Rat
- Omim: 606464 Human
- SwissProt: P81172 Human
- SwissProt: Q99MH3 Rat
- Unigene: 8821 Human
- Unigene: 7865 Rat
Anti-Hepcidin-25 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hepcidin-25 antibody (ab30760)
IHC image of ab30760 staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30760, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemistry (PFA fixed) - Hepcidin-25 antibody (ab30760)
Hepcidin-25 expression in Rat liver cells using Rabbit polyclonal to Hepcidin-25 (ab30760).Immunohistochemistry was performed on PFA fixed cells, ab30760 was used at 1/1000 incubated overnight at RT. Haemotoxylin is used as a nuclear counterstain in this image.
Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
Western blot - Hepcidin-25 antibody (ab30760)
Anti-Hepcidin-25 antibody (ab30760) at 1 µg/ml + HAMP Human Full-length Recombinant Protein (Tagged) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 3 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
ab30760 was tested against Human HAMP Full-length Recombinant Protein (Tagged) predicted to run at 32kDa.
Immunohistochemistry (Frozen sections) - Hepcidin-25 antibody (ab30760)
ab30760 at a 1/200 dilution staining Hepcidin-25 in human artery tissue sections by Immunohistochemistry (frozen sections) incubated for 24 hours at 20°C. Acetone fixed. Blocked with 0.5% BSA for 10 minutes at 20°C. Secondary used at 7µg/ml Goat anti-rabbit IgG (H+L) conjugated to biotin.Immunoperoxydase system and AEC were used to develop the color.
This image is courtesy of an anonymous abreview.
References for Anti-Hepcidin-25 antibody (ab30760)
This product has been referenced in:
- Bhatt Let al. Knockdown of beta2-microglobulin perturbs the subcellular distribution of HFE and hepcidin. Biochem Biophys Res Commun 378:727-31 (2009). ICC/IF; Human.Read more (PubMed: 19059216) »
See 1 publication for this product
Publishing research using ab30760? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"