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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hepcidin-25 antibody (ab30760)
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Immunohistochemistry (PFA fixed) - Hepcidin-25 antibody (ab30760)
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Western blot - Hepcidin-25 antibody (ab30760)
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Product Name
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Hepcidin-25 antibody
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See all Hepcidin-25 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Hepcidin-25
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 50 to the C-terminus of Human Hepcidin-25. (Peptide available as ab31875.)
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Reacts with
(species key)
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Hu, Rat
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Tested applications
(see key)
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IHC-Fr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IHC-P: Use at a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/200.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 3 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HAMP Human Full-length Recombinant Protein (Tagged)
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Cellular localization
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Secreted
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Research areas
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Cardiovascular >> Blood >> Erythrocytes
Signal Transduction >> Metabolism >> Vitamins / Minerals
Cardiovascular >> Blood >> Serum Proteins
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Relevance
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Hepcidin is the principal iron-regulatory hormone. It acts by binding to the iron exporter ferroportin, inducing its internalization and degradation, thereby blocking cellular iron efflux. The bioactive 25 amino acid (aa) peptide has a hairpin structure stabilized by 4 disulfide bonds. Pathogenic mutations in hepatic iron transporters and regulators lead to hereditary iron overload diseases, including hemochromatosis. The highest expression of hepcidin is found in the liver and is secreted into the urine.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.30 - 0.80 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Hepcidin-25 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Hepcidin-25 antibody images, references, products related to ab30760 and other tools.
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Hepcidin-25 antibody images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Hepcidin-25 antibody (ab30760)
IHC image of ab30760 staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab30760, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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Immunohistochemistry (PFA fixed) - Hepcidin-25 antibody (ab30760)
Hepcidin-25 expression in Rat liver cells using Rabbit polyclonal to Hepcidin-25 (ab30760).Immunohistochemistry was performed on PFA fixed cells, ab30760 was used at 1/1000 incubated overnight at RT. Haemotoxylin is used as a nuclear counterstain in this image. Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom
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Western blot - Hepcidin-25 antibody (ab30760)
Hepcidin-25 antibody (ab30760) at 1 µg/ml + HAMP Human Full-length Recombinant Protein (Tagged) at 0.1 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 3 kDa
Observed band size : 32 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutes
ab30760 was tested against Human HAMP Full-length Recombinant Protein (Tagged) predicted to run at 32kDa.
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Immunohistochemistry (Frozen sections) - Hepcidin-25 antibody (ab30760)
ab30760 at a 1/200 dilution staining Hepcidin-25 in human artery tissue sections by Immunohistochemistry (frozen sections) incubated for 24 hours at 20°C. Acetone fixed. Blocked with 0.5% BSA for 10 minutes at 20°C. Secondary used at 7µg/ml Goat anti-rabbit IgG (H+L) conjugated to biotin.Immunoperoxydase system and AEC were used to develop the color. This image is courtesy of an anonymous abreview.
See Abreview
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Search PubMed (MEDLINE) for references to Hepcidin-25
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Hepcidin-25 antibody - more information
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Customer reviews (feedback) regarding Hepcidin-25 antibody |
Customer FAQs regarding Hepcidin-25 antibody |
Protocols for Hepcidin-25 antibody |
Price and availability of products related to Hepcidin-25 antibody |
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Hepcidin-25 antibody - related products:
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Hepcidin-25 antibody - other tools:
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Contact Abcam with a Technical Enquiry about Hepcidin-25 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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