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Products:Signal Transduction >> Metabolism >> Vitamins / Minerals
Anti-Hephaestin antibody (ab56729)
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- Secondary antibodies
Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab56729? Please let us know so that we can cite the reference in this datasheet
ab56729 has been referenced in 1 publications.
- Yang WM et al. Transient expression of iron transport proteins in the capillary of the developing rat brain. Cell Mol Neurobiol 31:93-9 (2011). IHC-Fr; Rat. PubMed: 21061168
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - Anti-Hephaestin antibody (ab56729)
Predicted band size : 130 kDa
Hephaestin antibody (ab56729) at 1ug/lane + K-562 cell lysate at 25ug/lane.
Immunohistochemistry (Frozen sections) - Hephaestin antibody (ab56729)
ab56729 staining hephaestin (red) in Rat brain capillary endothelial cell tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1.5% serum for 1 hour at 20ºC. Samples were incubated with primary antibody (1/200 in PBS + 0.3% Triton X-100) for 12 hours at 4ºC. A Cy3®-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Prof. Dong Woon Kim
Immunocytochemistry/ Immunofluorescence - Anti-Hephaestin antibody (ab56729)
ICC/IF image of hepaestin staining on rat P3 glial culture using ab56729. The cells were fixed with 4% PFA in 0.1M PBS. Mature astrocytes are visible in red. Nucleus stained with Hoechst at high power magnification.
Western blot - Anti-Hephaestin antibody (ab56729)
All lanes : Anti-Hephaestin antibody (ab56729) at 1/1000 dilution
Lane 1 : Whole tissue lysate prepared from murine liver
Lane 2 : Whole tissue lysate prepared from murine liver
Lysates/proteins at 20 µg per lane.
Secondary
HRP conjugated sheep anti-mouse polyclonal at 1/5000 dilution
developed using the ECL technique
Predicted band size : 130 kDa
Exposure time : 1 minute
Image courtesy of Marco Spinazzi by Abreview.
Hephaestin antibody for Immunohistochemistry (Frozen sections) in Rat (56729)
Anti-Hephaestin antibody for Immunohistochemistry - Free Floating in Rat (56729)
Anti-Hephaestin antibody for Immunocytochemistry/ Immunofluorescence in Rat (56729)
Anti-Hephaestin antibody for Western blot in Mouse (56729)
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