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Anti-Hephaestin antibody
See all Hephaestin products (4) ...
Mouse monoclonal to Hephaestin
WB, IHC-Fr, IHC-FrFlmore details
Reacts with
Rat, Human
Recombinant fragment, corresponding to amino acids 315-425 of Human Hephaestin
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
PBS, pH 7.2
Concentration information loading...
Protein G purified
Monoclonal
IgG2a
kappa
Our Abpromise guarantee covers the use of ab56729 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 130 kDa.
IHC-Fr: Use at an assay dependent dilution.
IHC-FrFl: Use at an assay dependent concentration.
May function as a ferroxidase for ferrous (II) to ferric ion (III) conversion and may be involved in copper transport and homeostasis. Implicated in iron homeostasis and may mediate iron efflux associated to ferroportin 1.
Detected in breast, colon, bone trabecular cells and fibroblasts.
Belongs to the multicopper oxidase family.
Contains 6 plastocyanin-like domains.
Membrane.
Target information above from: UniProt accessionQ9BQS7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - Hephaestin antibody (ab56729)

Hephaestin antibody (ab56729) at 1ug/lane + K-562 cell lysate at 25ug/lane.
Immunohistochemistry (Frozen sections) - Hephaestin antibody (ab56729)

ab56729 staining hephaestin (red) in Rat brain capillary endothelial cell tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1.5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 0.3% Triton X-100) for 12 hours at 4°C. A Cy3®-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Prof. Dong Woon Kim
This product has been referenced in:
See 1 publication for this product
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Hephaestin antibody (ab56729) at 1ug/lane + K-562 cell lysate at 25ug/lane.

ab56729 staining hephaestin (red) in Rat brain capillary endothelial cell tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with Triton X-100 and blocked with 1.5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 0.3% Triton X-100) for 12 hours at 4°C. A Cy3®-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Prof. Dong Woon Kim
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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