Herring sperm ssDNA (ab46666)

Product Name 

Herring sperm ssDNA

Product type 

DNA RNA and cDNA

Description 

Herring sperm ssDNA

Tested applications 
(see key)

BL, ChIP, ISH

Application notes 
(see key)

Recommended dilutions
BL: Use at an assay dependent dilution.
ChIP: Use at an assay dependent dilution.
ISH: Use at an assay dependent dilution.

DNA for hybridization is used to block non-specific binding of probes to membranes. This product is prepared by sonication of purified herring sperm DNA. Sonication shears the large molecular weight DNA to produce fragments in a size range of 587 to 831 base pairs. This range has been shown to be the most effective for hybridizations. The material is monitored during sonication by electrophoresis in order to determine the size range. Once sonication is complete, the material is denatured by boiling.

Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.

In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. This product can be used as a blocking agent in Northern and Southern blotting as well as Chromatin Immunoprecipitation and other nucleic acid hybridization techniques.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> ChIP Related Products >> ChIP Related Products
Kits/ Lysates/ Other >> Tools and Reagents >> DNA

Relevance 

The cell death process during apoptosis is essential for normal tissue homeostasis and cellular differentiation in multi cellular organisms. Single stranded DNA (ss DNA) is produced by nuclear fragmentation during the early stages of the apoptotic process.

Purification notes 

This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.

Form 

Liquid

Concentration 

11.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

This is a ready to use concentrated solution (9-12 mg/ml DNA). However, it will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool ON ICE for at least 5 minutes prior to use. Cooling on ice will reduce the chances for reannealing, as it is more likely to reanneal if cooled at room temperature.

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