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Herring sperm ssDNA (ab46666)

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Overview

Product name

Herring sperm ssDNA

General notes

This is a ready to use concentrated solution (9-12 mg/ml DNA). However, it will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool ON ICE for at least 5 minutes prior to use. Cooling on ice will reduce the chances for reannealing, as it is more likely to reanneal if cooled at room temperature.

Tested applications

ChIP, in situ hybridisation, Blockingmore details

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Concentration

Concentration information loading...

Purification Notes

This DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.

Research areas

Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> Assays & Kits >> ChIP Related Products
Kits/ Lysates/ Other >> Tools and Reagents >> DNA
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA / Nucleotides

Relevance

The cell death process during apoptosis is essential for normal tissue homeostasis and cellular differentiation in multi cellular organisms. Single stranded DNA (ss DNA) is produced by nuclear fragmentation during the early stages of the apoptotic process.

Cellular localization

Nuclear

Alternative names

  • Single stranded deoxyribose nucleic acid
  • Single stranded DNA

Applications

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Our Abpromise guarantee covers the use of ab46666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Protocols

  • X-ChIP

    • References for Herring sperm ssDNA (ab46666)

    This product has been referenced in:

    • Godmann Met al. Epigenetic mechanisms regulate stem cell expressed genes Pou5f1 and Gfra1 in a male germ cell line. PLoS One 5:e12727 (2010). ChIP.Read more (PubMed: 20856864) »

    See 1 publication for this product

    Publishing research using ab46666? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"