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Overview

  • Product nameHerring sperm ssDNA
  • General notesThis is a ready to use concentrated solution (9-12 mg/ml DNA). However, it will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool ON ICE for at least 5 minutes prior to use. Cooling on ice will reduce the chances for reannealing, as it is more likely to reanneal if cooled at room temperature.
  • Tested applicationsChIP, In Situ Hybridisation, Blocking more details

    • Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Concentration information loading...
  • Purification NotesThis DNA is phenol-chloroform extracted, ethanol precipitated, and sonicated to produce single-stranded fragments which comigrate with the 587 and 831 base pair marker fragments.
  • Research Areas
  • RelevanceThe cell death process during apoptosis is essential for normal tissue homeostasis and cellular differentiation in multi cellular organisms. Single stranded DNA (ss DNA) is produced by nuclear fragmentation during the early stages of the apoptotic process.
  • Cellular localizationNuclear
    • Alternative names
        Single stranded deoxyribose nucleic acidSingle stranded DNA

    Applications

    Our Abpromise guarantee covers the use of ab46666 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ChIP ChIP: Use at an assay dependent dilution.
    In Situ Hybridisation ISH: Use at an assay dependent dilution.
    Blocking BL: Use at an assay dependent dilution.

    Protocols

    References for Herring sperm ssDNA (ab46666)

    This product has been referenced in:
    • Godmann M  et al. Epigenetic mechanisms regulate stem cell expressed genes Pou5f1 and Gfra1 in a male germ cell line. PLoS One 5:e12727 (2010). ChIP . Read more (PubMed: 20856864) »

    See 1 Publication for this product

    Publishing research using ab46666 ? Please let us know so that we can cite the reference in this datasheet

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    What is the formulation of this product?

    ab46666 is provided in pure water, it contains no buffer salts or EDTA. I hope this information helps, please do not hesitate to contact me should you have further questions.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"