Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-Hes1 antibody - Aminoterminal end
See all Hes1 products (8) ...
Description
Rabbit polyclonal to Hes1 - Aminoterminal end
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
A KLH conjugated synthetic peptide selected from the N-terminal region of human Hes1.
Positive control
HL60 cell line lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Ammonium Sulphate Precipitation
Purification notes
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - Hes1 antibody - Aminoterminal end (ab70933)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70933 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
Application notes
ELISA: 1/1000.
WB: 1/50 - 1/100. Detects a band of approximately 29 kDa plus a range of other bands. (predicted molecular weight: 29 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
Domain
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ14469
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
see all
Database links
- Entrez Gene: 3280 Human
- Omim: 139605 Human
- SwissProt: Q14469 Human
- Unigene: 250666 Human
Anti-Hes1 antibody - Aminoterminal end images:
Western blot - Hes1 antibody - Aminoterminal end (ab70933)
Anti-Hes1 antibody - Aminoterminal end (ab70933) at 1/60 dilution + HL60 cell line lysates at 35 µg
Predicted band size : 29 kDa
Observed band size : 29 kDa
References for Anti-Hes1 antibody - Aminoterminal end (ab70933)
ab70933 has not yet been referenced specifically in any publications.
Publishing research using ab70933? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"