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Western blot - Hes1 antibody - Aminoterminal end (ab70933)
Western blot - Hes1 antibody - Aminoterminal end (ab70933)
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Product Name 

Hes1 antibody - Aminoterminal end

 

See all Hes1 antibodies (4)...

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Hes1 - Aminoterminal end

Immunogen 

A KLH conjugated synthetic peptide selected from the N-terminal region of human Hes1.

Reacts with 
(species key)

Hu

Tested applications 
(see key)

ELISA, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
ELISA: 1/1000.
WB: 1/50 - 1/100. Detects a band of approximately 29 kDa plus a range of other bands. (predicted molecular weight: 29 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HL60 cell line lysates.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors
Chromatin and Nuclear Signaling >> Transcription >> Other factors
Neuroscience >> Neurology process >> Neurogenesis

Relevance 

Hes1 is a transcriptional repressor of genes that require a bHLH protein for their transcription. It may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Hes1 is expressed in developing neuroectodermal and endodermal endocrine tissues, and Hes1 deficient embryos show severe defects in neuronal development, as well as pancreatic hypoplasia.

 

Alternative names Hes1 antibody - Aminoterminal end (ab70933)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

3280    (Human)

Omim

    

139605    (Human)

SwissProt

    

Q14469    (Human)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Ammonium Sulphate Precipitation

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Hes1 antibody - Aminoterminal end is on this datasheet. But please do contact us if you would like any reassurance!


See below for Hes1 antibody - Aminoterminal end images, references, products related to ab70933 and other tools.

 

Hes1 antibody - Aminoterminal end images:

Product image  Western blot - Hes1 antibody - Aminoterminal end (ab70933)

Western blot - Hes1 antibody - Aminoterminal end (ab70933)

Hes1 antibody - Aminoterminal end (ab70933) at 1/60 dilution + HL60 cell line lysates at 35 µg

Predicted band size : 29 kDa
Observed band size : 29 kDa

 
 

Search PubMed (MEDLINE) for references to Hes1

 

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