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Western blot - Hes1 antibody - Aminoterminal end (ab70933)

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Product Name
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Hes1 antibody - Aminoterminal end
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See all Hes1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to Hes1 - Aminoterminal end
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Immunogen
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A KLH conjugated synthetic peptide selected from the N-terminal region of human Hes1.
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions ELISA: 1/1000. WB: 1/50 - 1/100. Detects a band of approximately 29 kDa plus a range of other bands. (predicted molecular weight: 29 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HL60 cell line lysates.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors Chromatin and Nuclear Signaling >> Transcription >> Other factors Neuroscience >> Neurology process >> Neurogenesis
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Relevance
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Hes1 is a transcriptional repressor of genes that require a bHLH protein for their transcription. It may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Hes1 is expressed in developing neuroectodermal and endodermal endocrine tissues, and Hes1 deficient embryos show severe defects in neuronal development, as well as pancreatic hypoplasia.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Ammonium Sulphate Precipitation
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Storage buffer
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Preservative: 0.09% Sodium Azide Constituents: PBS Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Purification notes
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This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Hes1 antibody - Aminoterminal end is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for Hes1 antibody - Aminoterminal end images, references, products related to ab70933 and other tools.
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Hes1 antibody - Aminoterminal end images:
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Western blot - Hes1 antibody - Aminoterminal end (ab70933)
Hes1 antibody - Aminoterminal end (ab70933) at 1/60 dilution + HL60 cell line lysates at 35 µg
Predicted band size : 29 kDa Observed band size : 29 kDa
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Search PubMed (MEDLINE) for references to Hes1
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Hes1 antibody - Aminoterminal end - more information
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