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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab49170? Please let us know so that we can cite the reference in this datasheet
ab49170 has been referenced in 1 publications.
- Kamstrup MR et al. Notch 1 as a potential therapeutic target in cutaneous T-cell lymphoma. Blood : (2010). WB; Human. PubMed: 20538790
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hes1 antibody (ab49170)
ab49170 (4µg/ml) staining Hes1 in human liver using an automated system (DAKO Autostainer Plus). Using this protocol there is weak cytoplasmic staining in plates of hepatic cells. However there is stronger nuclear staining in the plates of hepatic cells and hepatic arteries.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody (ab49170)
ICC/IF image of ab49170 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49170, 5µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Hes1 antibody (ab49170)
Anti-Hes1 antibody (ab49170) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 30 kDa
Additional bands at : 17 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
Western blot - Anti-Hes1 antibody (ab49170)
Predicted band size : 30 kDa
Western Blot using lysate prepared from Hes1 transfected Ls174 cells. ab49170 used at a 1µg/ml.
Western blot - Anti-Hes1 antibody (ab49170)
Predicted band size : 30 kDa
Western blot using lysate prepared from murine embryonic cells.ab49170 used at a 1/800 dilution and the secondary was used at a 1/5000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Hes1 antibody (ab49170)
ab49170 at 50µg/ml staining Hes1 in Ls174T cells by Immunocytochemistry/ Immunofluorescence.
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