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Anti-Hes1 antibody (ab49170)

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Overview

Product name

Anti-Hes1 antibody
See all Hes1 products (8) ...

Description

Rabbit polyclonal to Hes1

Tested applications

WB, IHC-P, ICC/IFmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Chicken, Cow, Pig, Zebrafish

Immunogen

A region within synthetic peptide: MPADIMEKNS SSPVAATPAS VNTTPDKPKT ASEHRKSSKP IMEKRRRARI, corresponding to internal sequence amino acids 1-50 of Human Hes1

MPADIMEKNS SSPVAATPAS VNTTPDKPKT ASEHRKSSKP IMEKRRRARI

Positive control

Raji cell lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Polyclonal

Isotype

IgG

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Hes1 antibody(ab49170)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Hes1 antibody(ab49170) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence-Hes1 antibody(ab49170)Immunocytochemistry/ Immunofluorescence-Hes1 antibody(ab49170) image (enlarge)

  • Western blot - Hes1 antibody (ab49170)Western blot - Hes1 antibody (ab49170) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab49170 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).

  • ShowHide1 Image

    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 4 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ShowHide1 Image

    ICC/IF

     ICC/IF: Use a concentrat...Read more →

    ICC/IF: Use a concentration of 5 µg/ml

Target

Function

Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.

Sequence similarities

Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.

Domain

Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ14469 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • bHLHb39 antibody
  • C-HAIRY1 antibody
  • c-hairy1A antibody
  • Class B basic helix-loop-helix protein 39 antibody
  • FLJ20408 antibody
  • Hairy and enhancer of split 1 (Drosophila) antibody
  • Hairy and enhancer of split 1 antibody
  • Hairy Enhancer of Split 1 antibody
  • Hairy homolog (Drosophila) antibody
  • Hairy Homolog antibody
  • Hairy like antibody
  • Hairy, Drosophila, homolog of antibody
  • Hairy-like protein antibody
  • Hairy/enhancer of split, Drosophila, homolog of, 1 antibody
  • HAIRY1 antibody
  • HES-1 antibody
  • hes1 antibody
  • Hes1 hairy and enhancer of split 1 (Drosophila) antibody
  • HES1_HUMAN antibody
  • HHL antibody
  • HL antibody
  • HRY antibody
  • MGC129109 antibody
  • OTTHUMP00000209031 antibody
  • RHL antibody
  • Transcription factor HES 1 antibody
  • Transcription factor HES-1 antibody
see all

Anti-Hes1 antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Hes1 antibody(ab49170)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Hes1 antibody(ab49170)

ab49170 (4µg/ml) staining Hes1 in human liver using an automated system (DAKO Autostainer Plus). Using this protocol there is weak cytoplasmic staining in plates of hepatic cells. However there is stronger nuclear staining in the plates of hepatic cells and hepatic arteries.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  Immunocytochemistry/ Immunofluorescence-Hes1 antibody(ab49170)

Immunocytochemistry/ Immunofluorescence-Hes1 antibody(ab49170)

ICC/IF image of ab49170 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49170, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Western blot - Hes1 antibody (ab49170)

Western blot - Hes1 antibody (ab49170)

Anti-Hes1 antibody (ab49170) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique

Performed under reducing conditions.

Predicted band size : 30 kDa
Observed band size : 30 kDa
Additional bands at : 17 kDa,70 kDa. We are unsure as to the identity of these extra bands.

Exposure time : 30 seconds

References for Anti-Hes1 antibody (ab49170)

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See 1 publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"