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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-Hes1 antibody
See all Hes1 products (8) ...
Description
Rabbit polyclonal to Hes1
Tested applications
WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Pig, Zebrafish
Immunogen
A region within synthetic peptide: MPADIMEKNS SSPVAATPAS VNTTPDKPKT ASEHRKSSKP IMEKRRRARI, corresponding to internal sequence amino acids 1-50 of Human Hes1
MPADIMEKNS SSPVAATPAS VNTTPDKPKT ASEHRKSSKP IMEKRRRARI
Positive control
Raji cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab49170 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 30 kDa (predicted molecular weight: 30 kDa).
IHC-P: Use a concentration of 4 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
Domain
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ14469
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
see all
Database links
- Entrez Gene: 395128 Chicken
- Entrez Gene: 539547 Cow
- Entrez Gene: 3280 Human
- Entrez Gene: 15205 Mouse
- Entrez Gene: 29577 Rat
- Omim: 139605 Human
- SwissProt: O57337 Chicken
- SwissProt: Q3ZBG4 Cow
see all
Anti-Hes1 antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Hes1 antibody(ab49170)
ab49170 (4µg/ml) staining Hes1 in human liver using an automated system (DAKO Autostainer Plus). Using this protocol there is weak cytoplasmic staining in plates of hepatic cells. However there is stronger nuclear staining in the plates of hepatic cells and hepatic arteries.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Immunocytochemistry/ Immunofluorescence-Hes1 antibody(ab49170)
ICC/IF image of ab49170 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49170, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Hes1 antibody (ab49170)
Anti-Hes1 antibody (ab49170) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 30 kDa
Additional bands at : 17 kDa,70 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 30 seconds
References for Anti-Hes1 antibody (ab49170)
This product has been referenced in:
- Kamstrup MRet al. Notch 1 as a potential therapeutic target in cutaneous T-cell lymphoma. Blood : (2010). WB; Human.Read more (PubMed: 20538790) »
See 1 publication for this product
Publishing research using ab49170? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"