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Anti-Hes1 antibody (ab55265)

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    2 questions for ab55265

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    Question 1

    Monday 28-November-2011

    Hi,

    A customer would like some advice as to what they can use as a negative control for hes1 with ab55265. The customer is doing Western Blot on nuclear extracts of T-ALL cell lines. The Abreviews recommend a –ve control for Flow Cyt but not for WB.

    I look forward to your feedback so that I can advise the customer, thanks.

    Kind regards,    

    ANSWER:

    		  

    Thank you for your enquiry.

    I am sorry to confirm that Hes1 is a transcription factor that is fairly ubiquitously expressed.  It is therefore difficult to suggest a negative control. Unfortunately, this antibody has been tested externally and a negative control was not used.

    I have conducted a literature search, but am regrettably not able to confirm a suitable negative control for Hes1. I can recommend to consider trying a more extensive literature search on the internet and Pubmed for more information.

    We aim to provide as much information as possible to customers, and I am sorry there are no more details to provide on this occasion.

    If you have any further questions, please do not hesitate to contact us.

    Question 2

    Tuesday 30-June-2009

    Does ab55265 Hef1 antibody detect Hes1 from the Notch signalling pathway?

    ANSWER:

    		  

    Thank you for your recent telephone enquiry.

    From a literature search, I can see that the Hes1 protein can be part of the notch signalling pathway. This particular publication states that NOTCH receptors can signal through the hairy and enhancer of split (HES)-1 transcriptional regulator:

    Diabetes. 2008 Sep;57(9):2413-20. HES-1 is involved in adaptation of adult human beta-cells to proliferation in vitro.Bar Y, Russ HA, Knoller S, Ouziel-Yahalom L, Efrat S.

    Link: http://www.ncbi.nlm.nih.gov/pubmed/18599525?ordinalpos=11&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum

    I hope this will be useful to you, and I can suggest undertaking a further literature search to confirm this and to obtain more information.

    According to our data, our external tester used the antibody to test some cell lysate (total protein extraction), but we have not especially used this antibody in our laboratories to research the Notch signalling pathway.

    I hope this information is helpful. Should you have any further questions, please do not hesitate to contact us.
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