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Products:Neuroscience >> Neurology process >> Neurogenesis
Overview
Product name
Anti-Hes1 antibody
See all Hes1 products (8) ...
Description
Mouse monoclonal to Hes1
Tested applications
WB, Flow Cytmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant fragment, corresponding to amino acids 36-143 of Human Hes1
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Isotype
IgG2b
Light chain type
kappa
Applications
Show applications key
Our Abpromise guarantee covers the use of ab55265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 30 kDa.
Flow Cyt: Use 1µg for 106 cells.
Target
Function
Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity.
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 Orange domain.
Domain
Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG).
The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho/TLE family members, transcriptional corepressors recruited to specific target DNA by Hairy-related proteins.
The bHLH, as well as cooperation between the central Orange domain and the C-terminal WRPW motif, is required for transcriptional repressor activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ14469
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- bHLHb39 antibody
- C-HAIRY1 antibody
- c-hairy1A antibody
see all
Database links
- Entrez Gene: 3280 Human
- Omim: 139605 Human
- SwissProt: Q14469 Human
- Unigene: 250666 Human
Anti-Hes1 antibody images:
Western blot - Hes1 antibody (ab55265)
Predicted band size : 30 kDa
Hes1 antibody (ab55265) at 1ug/lane + THP-1 cell lysate at 25ug/lane.
Flow Cytometry - Hes1 antibody (ab55265)
Overlay histogram showing HepG2 cells stained with ab55265 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55265, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 4% paraformaldehyde (10min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
References for Anti-Hes1 antibody (ab55265)
ab55265 has not yet been referenced specifically in any publications.
Publishing research using ab55265? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"